| http://purl.uniprot.org/citations/7828851 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7828851 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7828851 | http://www.w3.org/2000/01/rdf-schema#comment | "The CCAAT-binding factor is a conserved heteromeric transcription factor that binds to CCAAT box-containing upstream activation sites (UASs) within the promoters of numerous eukaryotic genes. The CCAAT-binding factor of Saccharomyces cerevisiae activates the transcription of these genes in response to growth in a nonfermentable carbon source. Previous studies have demonstrated that the HAP2, HAP3, and HAP4 subunits of the yeast CCAAT-binding factor are required for the transcriptional activation of genes containing a CCAAT box. Using the two-hybrid screening method, we have identified an additional component of the CCAAT-binding factor. We present the identification and characterization of a novel gene, HAP5, that encodes an additional subunit of the CCAAT-binding factor required for the assembly and DNA-binding activity of the complex. In a hap5 mutant, we show that CCAAT-binding activity is abolished in vitro. Furthermore, we demonstrate that purified recombinant HAP2, HAP3, and HAP5 are able to reconstitute CCAAT-binding activity in mobility shift analysis. These data suggest that the HAP2/3/5 heterotrimer represents a unique DNA-binding factor in which all three subunits of the complex are absolutely required for DNA-binding activity."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.9.1.47"xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.9.1.47"xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/author | "Guarente L."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/author | "Guarente L."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/author | "Xing Y."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/author | "Xing Y."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/author | "McNabb D.S."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/author | "McNabb D.S."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/pages | "47-58"xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/pages | "47-58"xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/title | "Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/title | "Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding."xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/7828851 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7828851 |
| http://purl.uniprot.org/citations/7828851 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7828851 |
| http://purl.uniprot.org/citations/7828851 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7828851 |
| http://purl.uniprot.org/citations/7828851 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7828851 |