http://purl.uniprot.org/citations/7830550 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/7830550 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/7830550 | http://www.w3.org/2000/01/rdf-schema#comment | "In this report we evaluate the role of catalase in the survival of Bordetella pertussis within human polymorphonuclear leukocytes (PMNs). Crude extracts of B. pertussis exhibited a single catalase activity when subjected to non-denaturing polyacrylamide gel electrophoresis and assayed for catalase activity. A plasmid containing B. pertussis katA was identified by complementation of UM255, a catalase-deficient strain of Escherichia coli. The nucleotide sequence of katA predicts a 55 kDa protein that shares homology with a class of haem-containing catalases found in both eubacteria and eukaryotes. Analysis of the nucleotide sequence upstream of katA revealed the presence of a copy of IS481, a B. pertussis-specific insertion sequence. The start site of transcription of katA was mapped to a T residue in IS481 by primer extension analysis performed with B. pertussis RNA and a katA-specific primer. A catalase-deficient strain of B. pertussis, DD900, was constructed by gene replacement. DD900 was more sensitive to killing by 1 and 5 mM H2O2 than the parental strain, BP339. However, there was no difference in the ability of DD900 and BP339 to survive for 2 h in human PMNs. This suggests that catalase plays no significant role in the survival of B. pertussis within PMNs."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.1994.tb01272.x"xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.1994.tb01272.x"xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/author | "DeShazer D."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/author | "DeShazer D."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/author | "Wood G.E."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/author | "Wood G.E."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/author | "Friedman R.L."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/author | "Friedman R.L."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/pages | "123-130"xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/pages | "123-130"xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/title | "Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/title | "Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence."xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/volume | "14"xsd:string |
http://purl.uniprot.org/citations/7830550 | http://purl.uniprot.org/core/volume | "14"xsd:string |
http://purl.uniprot.org/citations/7830550 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7830550 |
http://purl.uniprot.org/citations/7830550 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7830550 |
http://purl.uniprot.org/citations/7830550 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7830550 |
http://purl.uniprot.org/citations/7830550 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7830550 |