| http://purl.uniprot.org/citations/7835426 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7835426 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7835426 | http://www.w3.org/2000/01/rdf-schema#comment | "Recombinant purified Nef protein of HIV-1, as well as Nef protein derived from extracts of permanently HIV-1 infected glioblastoma cells and monocytes, are specifically cleaved by the HIV-1 protease. Nef cleavage products in cellular extracts treated with protease showed identical molecular weights as those obtained by digestion of purified Nef with recombinant HIV-1 protease. Since cellular extracts were prepared by detergent and mechanical lysis it cannot be excluded that physiological cytoplasmic conditions were altered. The lack of Nef cleavage by endogenous HIV-1 protease in infected cells might be due to low concentrations of viral protease and the presence of Gag precursor molecules as natural substrate. Using a panel of monoclonal antibodies two cleavage fragments of 19 kDa and 8 kDa were defined. The cleavage site was located by microsequencing between amino acid 57 and 58 (AW*LEAQEEEEVGF). The conserved cleavage motif within HIV-1 Nef suggests a potential biological function of Nef processing."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(94)01370-g"xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(94)01370-g"xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Schoen A."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Schoen A."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Erfle V."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Erfle V."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Gaedigk-Nitschko K."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Gaedigk-Nitschko K."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Kohleisen B."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Kohleisen B."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Wachinger G."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/author | "Wachinger G."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/pages | "275-278"xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/pages | "275-278"xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/title | "Cleavage of recombinant and cell derived human immunodeficiency virus 1 (HIV-1) Nef protein by HIV-1 protease."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/title | "Cleavage of recombinant and cell derived human immunodeficiency virus 1 (HIV-1) Nef protein by HIV-1 protease."xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/volume | "357"xsd:string |
| http://purl.uniprot.org/citations/7835426 | http://purl.uniprot.org/core/volume | "357"xsd:string |