| http://purl.uniprot.org/citations/7854121 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7854121 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7854121 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/7854121 | http://www.w3.org/2000/01/rdf-schema#comment | "Cloning and nucleotide sequencing indicated that transposon Tn1546 from Enterococcus faecium BM4147 encodes a 23,365 Da protein, VanX, required for glycopeptide resistance. The vanX gene was located downstream from genes encoding the VanA ligase and the VanH dehydrogenase which synthesize the depsipeptide D-alanyl-D-lactate (D-Ala-D-Lac). In the presence of ramoplanin, an Enterococcus faecalis JH2-2 derivative producing VanH, VanA and VanX accumulated mainly UDP-MurNAc-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Lac (pentadepsipeptide) and small amounts of UDP-MurNAc-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala (pentapeptide) in the ratio 49:1. Insertional inactivation of vanX led to increased synthesis of pentapeptide with a resulting change in the ratio of pentadepsipeptide: pentapeptide to less than 1:1. Expression of vanX in E. faecalis and Escherichia coli resulted in production of a D,D-dipeptidase that hydrolysed D-Ala-D-Ala. Pentadepsipeptide, pentapeptide and D-Ala-D-Lac were not substrates for the enzyme. These results establish that VanX is required for production of a D,D-dipeptidase that hydrolyses D-Ala-D-Ala, thereby preventing pentapeptide synthesis and subsequent binding of glycopeptides to D-Ala-D-Ala-containing peptidoglycan precursors at the cell surface."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.1994.tb00497.x"xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.1994.tb00497.x"xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Courvalin P."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Courvalin P."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Arthur M."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Arthur M."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Dutka-Malen S."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Dutka-Malen S."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Reynolds P.E."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Reynolds P.E."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Depardieu F."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/author | "Depardieu F."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/pages | "1065-1070"xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/pages | "1065-1070"xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/title | "Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/title | "Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine."xsd:string |
| http://purl.uniprot.org/citations/7854121 | http://purl.uniprot.org/core/volume | "13"xsd:string |