| http://purl.uniprot.org/citations/7876099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7876099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7876099 | http://www.w3.org/2000/01/rdf-schema#comment | "A full-length 1966-base pair clone of the human class IV alcohol dehydrogenase (sigma-ADH) was isolated from a human stomach cDNA library. The 373-amino acid sigma-ADH encoded by this cDNA was expressed in Escherichia coli. The specific activity of the recombinant enzyme for ethanol oxidation at pH 7.5 and 25 degrees C, calculated from active-site titration of NADH binding, was 92 +/-9 units/mg. Kinetic analysis of the catalytic efficiency (kcat/KM) of recombinant sigma-ADH for oxidation of primary alcohols indicated broad substrate specificity. Recombinant human sigma-ADH exhibited high catalytic efficiency for oxidation of all-trans-retinol to all-trans-retinal. This pathway is important in the synthesis of the transcriptional regulator all-trans-retinoic acid. Secondary alcohols and 3 beta-hydroxysteroids were inactive with sigma-ADH or were oxidized with very low efficiency. The KM of sigma-ADH for ethanol was 25 mM, and the KM for primary straight chain alcohols decreased substantially as chain length increased. There are important amino acid differences in the alcohol-binding site between the human class IV (sigma) and human class I (beta) alcohol dehydrogenases that appear to explain the high catalytic efficiency for all-trans-retinol, the high kcat for ethanol, and the low catalytic efficiency for secondary alcohols of sigma-ADH relative to beta 1-ADH. For example, modeling the binding of all-trans-retinol in the human beta 1-ADH structure suggested that coordination of retinol to the active-site zinc is hindered by a loop from residues 114 to 120 that is at the entrance to the alcohol-binding site. The deletion of Gly-117 in human sigma-ADH and a substitution of Leu for the bulky Tyr-110 appear to facilitate retinol access to the active-site zinc."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.8.3625"xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.8.3625"xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Edenberg H.J."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Edenberg H.J."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Bosron W.F."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Bosron W.F."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Carr L.G."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Carr L.G."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Hurley T.D."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Hurley T.D."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Kedishvili N.Y."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Kedishvili N.Y."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Li T.K."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Li T.K."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Stone C.L."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Stone C.L."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Peggs C.F."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Peggs C.F."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Popov K.M."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Popov K.M."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Thomasson H.R."xsd:string |
| http://purl.uniprot.org/citations/7876099 | http://purl.uniprot.org/core/author | "Thomasson H.R."xsd:string |