| http://purl.uniprot.org/citations/7890754 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7890754 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7890754 | http://www.w3.org/2000/01/rdf-schema#comment | "An azidoubiquinone derivative, 3-azido-2-methyl-5-methoxy [3H]-6-decyl-1,4-benzoquinone ([3H]azido-Q), was used to study the ubiquinone-protein interaction and to identify ubiquinone-binding proteins in bovine heart mitochondrial succinate-ubiquinone reductase. When the reductase was incubated with [3H]azido-Q and illuminated with long wavelength UV light, the decrease in the enzymatic activity correlated with the amount of azido-Q incorporated into the protein. When the illuminated, [3H]azido-Q-treated reductase was extracted with organic solvent and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, radioactivity was found primarily in the QPs1 subunit. The [3H]azido-Q-labeled QPs1 was purified from labeled reductase by a procedure involving ammonium sulfate fractionation, dialysis, organic solvent extraction, lyophilization, preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and cold acetone precipitation. The purified, [3H]azido-Q-labeled QPs1 protein was subjected to reductive carboxymethylation prior to digestion by trypsin. One azido-Q-linked peptide, with a retention time of 66.9 min, was obtained by high performance liquid chromatographic separation. The partial amino-terminal sequence of this peptide is GLTISQL-, indicating that this tryptic peptide comprises amino acid residues 113-140 of the revised amino acid sequence of QPs1. The Q-binding domain, using the proposed structure of QPs1, is probably located in the stretch connecting transmembrane helices 2 and 3 that extrude from the surface of the M side of the inner membrane."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.11.6193"xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.11.6193"xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "Yu L."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "Yu L."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "Lee G.Y."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "Lee G.Y."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "He D.Y."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "He D.Y."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "Yu C.A."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/author | "Yu C.A."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/pages | "6193-6198"xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/pages | "6193-6198"xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/title | "Identification of the ubiquinone-binding domain in QPs1 of succinate-ubiquinone reductase."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/title | "Identification of the ubiquinone-binding domain in QPs1 of succinate-ubiquinone reductase."xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7890754 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7890754 |
| http://purl.uniprot.org/citations/7890754 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7890754 |