| http://purl.uniprot.org/citations/7901228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7901228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7901228 | http://www.w3.org/2000/01/rdf-schema#comment | "The murine Hoxa-4 gene encodes a protein with a homeodomain closely related to those produced by the Antennapedia-like class of Drosophila genes. Drosophila homeodomain proteins can function as transcription factors, binding to several specific DNA sequences. One sequence that is frequently encountered contains a core ATTA motif within a larger consensus sequence, such as CAATTAA. The in vitro synthesized protein product of Hoxa-4 was shown to bind to a subset of restriction fragments of the Hoxa-4 gene itself as determined by gel retardation experiments. Direct examination of the sequences of the fragments bound by Hoxa-4 protein revealed the presence of four regions containing the core ATTA motif. Two regions contained sequences of the CAATTAA class and were located approximately 1 kb upstream from the putative somatic Hoxa-4 promoter and within the intron. Two additional binding sites containing the consensus target sequence involved in autoregulation of Drosophila Deformed gene were identified: one immediately downstream of the putative embryonic transcription start site and one within the intron, respectively. Specific binding of the in vitro produced Hoxa-4 protein to oligonucleotides corresponding to these sequences was observed in gel retardation assays. The same results were obtained with Hoxa-4 protein produced in a Baculovirus expression system. Experiments using oligonucleotides containing base substitutions in positions 1, 3, 4, and 5 in the sequence CAATTAA showed severely reduced binding. The use of truncated mutant Hoxa-4 proteins in gel retardation assays and in transient co-transfection experiments revealed that the intact homeodomain was required for the binding. These results also suggested that the Hoxa-4 gene has the potential to auto-regulate its expression by interacting with the homeodomain binding sites present in the promoter as well as in the intron."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.240520409"xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.240520409"xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/author | "Wu K."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/author | "Wu K."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/author | "Wolgemuth D.J."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/author | "Wolgemuth D.J."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/name | "J. Cell. Biochem."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/name | "J. Cell. Biochem."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/pages | "449-462"xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/pages | "449-462"xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/title | "Protein product of the somatic-type transcript of the Hoxa-4 (Hox-1.4) gene binds to homeobox consensus binding sites in its promoter and intron."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/title | "Protein product of the somatic-type transcript of the Hoxa-4 (Hox-1.4) gene binds to homeobox consensus binding sites in its promoter and intron."xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/volume | "52"xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://purl.uniprot.org/core/volume | "52"xsd:string |
| http://purl.uniprot.org/citations/7901228 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7901228 |
| http://purl.uniprot.org/citations/7901228 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7901228 |
| http://purl.uniprot.org/citations/7901228 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7901228 |
| http://purl.uniprot.org/citations/7901228 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7901228 |
| http://purl.uniprot.org/uniprot/P06798 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7901228 |
| http://purl.uniprot.org/uniprot/P06798#SIP008DA86B792E17A0 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7901228 |