| http://purl.uniprot.org/citations/7916611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7916611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7916611 | http://www.w3.org/2000/01/rdf-schema#comment | "A synthetic gene encoding the peptide sequence for the low molecular weight (M(r) approximately 9600 Da) high-potential iron protein (HiPIP) from the photosynthetic bacterium Chromatium vinosum has been constructed by shotgun ligation of twelve complimentary oligonucleotides varying in size from 42-mers to 48-mers. After cloning the gene into a pET-21d(+) vector, expression of holoprotein in yields of 35 mg/liter of culture was obtained following induction with isopropyl-beta-D-thiogalactoside (IPTG). The recombinant protein was characterized by electronic absorption, 1H NMR, electrochemistry, N-terminal sequencing and amino acid analysis. This is the first example of the expression of a high potential ferredoxin containing a fully constituted [Fe4S4] cluster."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1993.2626"xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1993.2626"xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Agarwal A."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Agarwal A."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Tan J."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Tan J."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Cowan J.A."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Cowan J.A."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Lui S.M."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Lui S.M."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Eren M."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Eren M."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Tevelev A."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/author | "Tevelev A."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/pages | "1357-1362"xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/pages | "1357-1362"xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/title | "Synthesis, cloning and expression of a synthetic gene for high potential iron protein from Chromatium vinosum."xsd:string |
| http://purl.uniprot.org/citations/7916611 | http://purl.uniprot.org/core/title | "Synthesis, cloning and expression of a synthetic gene for high potential iron protein from Chromatium vinosum."xsd:string |