Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/7922031http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/7922031http://www.w3.org/2000/01/rdf-schema#comment"

Background

Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. It is a member of a family of glycoprotein hormones that are disulfide-rich heterodimers, with a common alpha-chain and distinctive beta-chains specific to their particular G-protein linked receptors.

Results

We have produced recombinant hCG in mammalian cells as the selenomethionyl protein, and have determined its structure (after partial deglycosylation) at 2.6 A resolution from multiwavelength anomalous diffraction (MAD) measurements. Despite only limited sequence similarity (10% identity), the alpha- and beta-subunits of hCG have similar tertiary folds. Each subunit has a cystine-knot motif at its core of extended hairpin loops. There is a very extensive subunit interface featuring two inter-chain beta-sheets and a unique, disulfide-tethered 'arm' from the beta-subunit which 'embraces' the alpha-subunit. The carboxy-terminal peptide of the beta-subunit, which is rich in O-linked sugars, is disordered.

Conclusions

Structural and sequence comparisons indicate an evolutionary homology, albeit remote, between the glycoprotein hormone chains and other cystine-knot proteins, notably platelet-derived growth factor. Segments of the alpha- and beta-chains that have been convincingly implicated in receptor binding by hCG are juxtaposed on one side of the molecule. A glycosylation site implicated in signal transduction but not in binding is also close to the presumed binding site suggesting a possible coupling between ligand binding and signaling. This study with selenomethionyl protein produced in mammalian cells extends the realm of MAD phasing."xsd:string
http://purl.uniprot.org/citations/7922031http://purl.org/dc/terms/identifier"doi:10.1016/s0969-2126(00)00054-x"xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/author"Liu Y."xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/author"Wu H."xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/author"Hendrickson W.A."xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/author"Canfield R.E."xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/author"Lustbader J.W."xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/name"Structure"xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/pages"545-558"xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/title"Structure of human chorionic gonadotropin at 2.6 A resolution from MAD analysis of the selenomethionyl protein."xsd:string
http://purl.uniprot.org/citations/7922031http://purl.uniprot.org/core/volume"2"xsd:string
http://purl.uniprot.org/citations/7922031http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/7922031
http://purl.uniprot.org/citations/7922031http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/7922031
http://purl.uniprot.org/uniprot/P0DN86#attribution-66752ADFD87A36432754586AC93556E6http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/P0DN86#attribution-83649A013FC120C04038D69C57DCFFB8http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/P01215#attribution-66752ADFD87A36432754586AC93556E6http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/P01215#attribution-83649A013FC120C04038D69C57DCFFB8http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/#_P01660-mappedCitation-7922031http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/#_P01750-mappedCitation-7922031http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/#_P18526-mappedCitation-7922031http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/#_P0DN86-mappedCitation-7922031http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/#_P01215-mappedCitation-7922031http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/7922031
http://purl.uniprot.org/uniprot/P0DN86http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/7922031
▶