| http://purl.uniprot.org/citations/7929063 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7929063 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7929063 | http://www.w3.org/2000/01/rdf-schema#comment | "Three subunits, Ac115, Ac39, and the proteolipid, were positively identified in the membrane sectors of V-ATPases from different sources. We searched for organelle-specific protein in purified preparations of V-ATPase from bovine chromaffin granules. A diffused protein band at a position of about 45 kDa was identified in SDS-polyacrylamide gels of the above preparation. Following digestion with endopeptidase Glu-C (V-8), a polypeptide of about 10 kDa was isolated and subjected to amino acid sequencing. Hence, the cDNA encoding the protein Ac45 was cloned from a bovine adrenal medulla library. The cDNA sequence contains an open reading frame encoding a protein of 468 amino acids with a calculated molecular mass of 51,786 daltons. A potential signal sequence comprised of the first 35 amino acids and a potential transmembrane domain at the C terminus of the protein were identified. There exist seven potential glycosylation sites between the aforementioned protein motifs. Experiments with a specific antibody against Ac45 demonstrated that it is copurifying with the V-ATPase from chromaffin granules. Immunological cross-reactivity was observed with purified V-ATPase from bovine kidney microsomes but not from plasma membranes of epithelial cells. Cell-free expression of the protein from synthetic mRNA produced a single protein band at about 50 kDa on SDS gels. Upon inclusion of dog pancreas microsomes in the reaction mixture, a slow migrating band sensitive to peptide:N-glycosidase F was observed."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)51053-5"xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)51053-5"xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Nelson N."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Nelson N."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Pan Y.-C.E."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Pan Y.-C.E."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Mandiyan S."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Mandiyan S."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Nelson H."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Nelson H."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Supek F."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Supek F."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Supekova L."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/author | "Supekova L."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/pages | "24102-24106"xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/pages | "24102-24106"xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/title | "A novel accessory subunit for vacuolar H(+)-ATPase from chromaffin granules."xsd:string |
| http://purl.uniprot.org/citations/7929063 | http://purl.uniprot.org/core/title | "A novel accessory subunit for vacuolar H(+)-ATPase from chromaffin granules."xsd:string |