| http://purl.uniprot.org/citations/7937966 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7937966 | http://www.w3.org/2000/01/rdf-schema#comment | "A jellyroll beta-sandwich protein, the Bacillus beta-glucanase H(A16-M), is used to probe the role of N-terminal peptide regions in protein folding in vivo. A gene encoding H(A16-M) is rearranged to place residues 1-58 of the protein behind a signal peptide and residues 59-214. The rearranged gene is expressed in Escherichia coli. The resultant circularly permuted protein, cpA16M-59, is secreted into the periplasm, correctly processed, and folded into a stable and active enzyme. Crystal structure analysis at 2.0-A resolution, R = 15.3%, shows cpA16M-59 to have a three-dimensional structure nearly identical with that of the parent beta-glucanase. An analogous experiment based on the wild-type Bacillus macerans beta-glucanase, giving rise to the circularly permuted variant cpMAC-57, yields the same results. Folding of these proteins, therefore, is not a vectorial process depending on the conformation adopted by their native N-terminal oligopeptides after ribosomal synthesis and translocation through the cytoplasmic membrane."xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.91.22.10417"xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/author | "Borriss R."xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/author | "Piotukh K."xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/author | "Hahn M."xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/author | "Heinemann U."xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/pages | "10417-10421"xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/title | "Native-like in vivo folding of a circularly permuted jellyroll protein shown by crystal structure analysis."xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://purl.uniprot.org/core/volume | "91"xsd:string |
| http://purl.uniprot.org/citations/7937966 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7937966 |
| http://purl.uniprot.org/citations/7937966 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7937966 |
| http://purl.uniprot.org/uniprot/#_P23904-mappedCitation-7937966 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/7937966 |
| http://purl.uniprot.org/uniprot/P23904 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/7937966 |