| http://purl.uniprot.org/citations/7969037 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7969037 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7969037 | http://www.w3.org/2000/01/rdf-schema#comment | "omega-Agatoxin-TK (omega-Aga-TK), a 48-amino-acid peptide isolated from the venom of the funnel web spider (Agelenopsis aperta), is a selective and potent inhibitor of P-type calcium channels in the nervous system. We have synthesized a peptide that has the amino acid sequence identified for native omega-Aga-TK. The synthetic omega-Aga-TK, however, showed 80-90-fold less potent inhibition of P-type calcium channels, compared with native omega-Aga-TK. Enantiomer analysis of native omega-Aga-TK revealed D-serine at position 46, and synthetic omega-[D-Ser46]Aga-TK had the same potency as native omega-Aga-TK for blocking P-type calcium channels in cultured cerebellar Purkinje neurons. Two peptide fragments of omega-Aga-TK, namely omega-Aga-TK(1-43) and the carboxyl-terminal peptide fragment omega-Aga-TK(44-48), did not produce any significant inhibition of P-type calcium channels or interfere with the blockade of the channels elicited by native omega-Aga-TK. Molecular dynamics calculations showed that the carboxyl-terminal, six-amino-acid peptide of omega-Aga-TK containing D-Ser46 assumes a different conformation than does the peptide containing L-Ser46. These results suggest that the specific conformation of the carboxyl-terminal region of omega-Aga-TK, particularly the configuration of Ser46, together with a beta-sheet structure formed by four disulfide bonds, might be essential for blockade of P-type calcium channels."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kawai T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kawai T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Watanabe T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Watanabe T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Nishizawa Y."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Nishizawa Y."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Teramoto T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Teramoto T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Sawada K."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Sawada K."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Nakajima K."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Nakajima K."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kawakami Y."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kawakami Y."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Niidome T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Niidome T."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kuwada M."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kuwada M."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kumagaye K.Y."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/author | "Kumagaye K.Y."xsd:string |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7969037 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |