| http://purl.uniprot.org/citations/8006084 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8006084 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8006084 | http://www.w3.org/2000/01/rdf-schema#comment | "A Drosophila cDNA (BAD1) encoding a structural and assembly-competent homologue of the mammalian coated pit beta-adaptins (beta and beta') has been cloned and sequenced. In its amino-terminal region (residues 1-575), the BAD1 sequence appears intermediate between that of the mammalian beta-adaptin and a predicted sequence, from cDNA 105a, which appears to code for a version of beta'-adaptin. To test its functional characteristics, a 'myc'-tagged version of BAD1 was expressed in Cos cells. The BAD1 protein was detected most clearly in plasma membrane coated pits, where it colocalized with alpha-adaptin, although other coated pits were noted which apparently did not contain alpha-adaptin. However, these are probably gamma-adaptin containing pits, as BAD1 was also found colocalized with gamma-adaptin in Golgi coated pits in which, typically, alpha-adaptin is absent. Immunoprecipitation experiments confirmed that the BAD1 protein was present in both types of adaptor complex, unlike beta-adaptin which complexes with alpha-adaptin and beta'-adaptin which partners gamma-adaptin exclusively. In spite of this, BAD1 expression does not appear to mix alpha-adaptin and gamma-adaptin distribution amongst all the coated pits: thus the location of these adaptor complexes in mammalian cells does not depend on the differences between beta subunits but rather on membrane-specific interactions of other adaptor polypeptides. The differential interaction of beta with alpha-adaptin and beta' with gamma-adaptin in mammalian cells is likely to depend on the few non-conservative differences between their respective sequences and BAD1. Four of these (one with respect to beta and three versus 105a) are clustered in a particular region (residues 155 to 305), which may therefore represent a domain that influences the choice of partner adaptin."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/author | "Pearse B.M."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/author | "Pearse B.M."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/author | "Camidge D.R."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/author | "Camidge D.R."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/pages | "709-718"xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/pages | "709-718"xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/title | "Cloning of Drosophila beta-adaptin and its localization on expression in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/title | "Cloning of Drosophila beta-adaptin and its localization on expression in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/volume | "107"xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://purl.uniprot.org/core/volume | "107"xsd:string |
| http://purl.uniprot.org/citations/8006084 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8006084 |
| http://purl.uniprot.org/citations/8006084 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8006084 |
| http://purl.uniprot.org/citations/8006084 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8006084 |
| http://purl.uniprot.org/citations/8006084 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8006084 |
| http://purl.uniprot.org/uniprot/Q24253 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/8006084 |
| http://purl.uniprot.org/uniprot/Q24253#attribution-5219672BD5A5F2458CC65F31E17DB1DA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8006084 |
| http://purl.uniprot.org/uniprot/Q24253#attribution-96C2BD99FF160D9E25D954F66C079853 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8006084 |
| http://purl.uniprot.org/uniprot/Q960F2#attribution-5BA542CABC1E63CD05879675375803C8 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8006084 |