| http://purl.uniprot.org/citations/8051010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8051010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8051010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8051010 | http://www.w3.org/2000/01/rdf-schema#comment | "Successful completion of the bacteriophage P2 lytic cycle requires phage-induced lysis of its Escherichia coli host, a process that is poorly understood. Genetic analysis of lysis-deficient mutants defined a single locus, gene K, which lies within the largest late transcription unit of P2 and maps between head gene L and tail gene R. We determined and analyzed the DNA sequence of a ca. 2.1-kb EcoRV fragment that spans the entire region from L to R, thus completing the sequence of this operon. This region contains all of the functions necessary for host cell lysis. Sequence analysis revealed five open reading frames, initially designated orf19 through orf23. All of the existing lysis mutants--ts60, am12, am76, and am218--were located in orf21, which must therefore correspond to gene K. The K gene product has extensive amino acid sequence similarity to the product of gene R of bacteriophage lambda, and its exhibits endolysin function. Site-directed mutagenesis and reverse genetics were used to create P2 amber mutants in each of the four other newly identified open reading frames. Both orf19 (gene X) and orf20 (gene Y) encode essential functions, whereas orf22 (lysA) and orf23 (lysB) are nonessential. Gene Y encodes a polypeptide with striking similarities to the family of holin proteins exemplified by gpS of phage lambda, and the Yam mutant displayed the expected properties of a holin mutant. The gene products of lysA and lysB, although nonessential, appear to play a role in the correct timing of lysis, since a lysA amber mutant caused slightly accelerated lysis and a lysB amber mutant slightly delayed lysis of nonpermissive strains. Gene X must encode a tail protein, since lysates from nonpermissive cells infected with the X amber mutant were complemented in vitro by similar lysates of cells infected with P2 head mutants but not with tail mutants."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.176.16.4974-4984.1994"xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.176.16.4974-4984.1994"xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Bartlett B."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Bartlett B."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Bartlett B."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Calendar R."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Calendar R."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Calendar R."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Christie G.E."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Christie G.E."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Christie G.E."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Ziermann R."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Ziermann R."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/author | "Ziermann R."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/name | "J. Bact."xsd:string |
| http://purl.uniprot.org/citations/8051010 | http://purl.uniprot.org/core/pages | "4974-4984"xsd:string |