| http://purl.uniprot.org/citations/8087204 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8087204 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8087204 | http://www.w3.org/2000/01/rdf-schema#comment | "Two low molecular weight fibrinolytic/hemorrhagic enzymes, jararafibrase III and jararafibrase IV, were purified from Bothrops jararaca venom using a fast protein liquid chromatography system. The purified jararafibrase III and jararafibrase IV were single chain proteins with molecular weights of 20,400 +/-500 and 21,200 +/-400, respectively, by SDS-PAGE. The isoelectric points of jararafibrase III and jararafibrase IV were 9.4 and 6.9, respectively. The activity of the enzyme was inhibited by 1,10-phenanthroline and EDTA, suggesting that both enzymes were metalloproteinases. The specific fibrinolytic activities of jararafibrase III and jararafibrase IV were 7.5 +/- 0.4 and 6.5 +/-1.6 units/mg protein, respectively. The enzymes induced local hemorrhage in the skin of rats. The minimal hemorrhagic doses of jararafibrase III and IV were 31.0 and 34.0 micrograms/rat, respectively. The enzymes displayed broad substrate specificities like the previously purified jararafibrases I and II. Jararabrases III and IV degraded type-IV collagen, gelatin, laminin and fibronectin into smaller fragments. The specific activities of jararafibrase III for type-IV collagen and gelatin were 7.6 +/- 0.3 and 43 +/-11 units/mg protein, respectively. The specific activities of jararafibrase IV for type IV-collagen and gelatin were 16.5 +/- 1.2 and 112 +/-9 units/mg protein, respectively."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.org/dc/terms/identifier | "doi:10.1159/000468668"xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.org/dc/terms/identifier | "doi:10.1159/000468668"xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Tanigawa M."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Tanigawa M."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Yoshida E."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Yoshida E."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Maruyama M."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Maruyama M."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Mihara H."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Mihara H."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Sugiki M."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/author | "Sugiki M."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/name | "Enzyme Protein"xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/name | "Enzyme Protein"xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/pages | "124-135"xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/pages | "124-135"xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/title | "Purification and characterization of low molecular weight fibrinolytic/hemorrhagic enzymes from snake (Bothrops jararaca) venom."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/title | "Purification and characterization of low molecular weight fibrinolytic/hemorrhagic enzymes from snake (Bothrops jararaca) venom."xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/8087204 | http://purl.uniprot.org/core/volume | "47"xsd:string |