| http://purl.uniprot.org/citations/8099279 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8099279 | http://www.w3.org/2000/01/rdf-schema#comment | "Previous studies have established that the human colon carcinoma cell line HT29 expresses an alpha 2-adrenergic receptor of the alpha 2A subtype, which is negatively coupled to adenylate cyclase. The purpose of the present study was to examine the mechanisms of alpha 2-adrenergic signal transduction in these cells. [32P]ADP-ribosylation with pertussis toxin and immunoblots using antibodies specific for the Gi alpha-subunits indicated that two distinct Gi-proteins (Gi2 and Gi3) were present in HT29-cell membranes. Treatment of intact cells with pertussis toxin resulted in a time-dependent decrease in the amount of [32P]ADP-ribosylatable Gi2 and Gi3, which coincided with a diminution in the number of alpha 2-adrenergic receptors in high-affinity state for agonists and with a progressive loss of ability of UK14304 to inhibit forskolin-stimulated accumulation of cyclic AMP. When membranes were [32P]ADP-ribosylated with cholera toxin in the absence of exogenous added guanine nucleotides, radioactivity was incorporated into a 45 kDa polypeptide representing Gs, as well as into 40-41 kDa polypeptides corresponding to Gi3 and Gi2. The amount of radioactivity incorporated into the two GiS under basal conditions was decreased by addition of the alpha 2-antagonist RX821002. It was not significantly affected by addition of clonidine (partial alpha 2-agonist), but was doubled by the addition of UK14304 (full alpha 2-agonist). This effect was blocked by RX821002. Study of adenylate cyclase activity indicated that preincubation of HT29 membranes with the antibody AS/7 (anti-alpha i1/alpha i2), but not with the antibody EC/2 (anti-alpha i3), attenuated the inhibitory effect of UK14304 on forskolin-stimulated adenylate cyclase. These data demonstrate that the alpha 2A-adrenergic receptor is coupled to both Gi2 and Gi3, and identify Gi2 as the major mediator of inhibition of adenylate cyclase in HT29 cells."xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2920283"xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/author | "Paris H."xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/author | "Daviaud D."xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/author | "Rouot B."xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/author | "Larrouy D."xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/author | "Remaury A."xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/pages | "283-288"xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/title | "Coupling of the alpha 2-adrenergic receptor to the inhibitory G-protein Gi and adenylate cyclase in HT29 cells."xsd:string |
| http://purl.uniprot.org/citations/8099279 | http://purl.uniprot.org/core/volume | "292"xsd:string |
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