RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/8113171http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8113171http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8113171http://www.w3.org/2000/01/rdf-schema#comment"The rpoN gene of Pseudomonas aeruginosa is required for the expression of a number of diverse genes, ranging from several classes of bacterial adhesins to enzymes for amino acid biosynthesis. The nucleotide sequence of the rpoN gene and its flanking region has been determined. The deduced amino acid sequence of the rpoN product is highly homologous to sequences of RpoN proteins of other microorganisms. Moreover, two open reading frames (ORF1 and ORF2) encoding peptides of 103 and 154 amino acids long, respectively, were found downstream of the rpoN gene. These two ORF products have a high degree of amino acid sequence homology with products of similar ORFs located adjacent to the rpoN genes in other microorganisms. Mutations in either ORF lead to a significant increase in P. aeruginosa generation time when propagated on minimal medium. These mutations had no effect on the expression of pilin or flagellin genes, whose expression depends on RpoN. Complementation analysis showed that the two ORFs are in the same transcriptional unit and the growth defects of the two ORF mutants on minimal medium are due to mutational effects on ORF2. The adverse effect of the ORF mutations on the growth of P. aeruginosa in minimal media can be suppressed by the addition of glutamine but not arginine, glutamate, histidine, or proline. Since rpoN mutants of P. aeruginosa display this same amino acid requirement for growth, the ORF2 product very likely functions as a coinducer of some but not all of the RpoN-controlled genes."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.org/dc/terms/identifier"doi:10.1128/jb.176.5.1316-1322.1994"xsd:string
http://purl.uniprot.org/citations/8113171http://purl.org/dc/terms/identifier"doi:10.1128/jb.176.5.1316-1322.1994"xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/author"Jin S."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/author"Jin S."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/author"Lory S."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/author"Lory S."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/author"Ishimoto K."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/author"Ishimoto K."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/pages"1316-1322"xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/pages"1316-1322"xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/title"Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/title"Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa."xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/volume"176"xsd:string
http://purl.uniprot.org/citations/8113171http://purl.uniprot.org/core/volume"176"xsd:string
http://purl.uniprot.org/citations/8113171http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8113171
http://purl.uniprot.org/citations/8113171http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8113171
http://purl.uniprot.org/citations/8113171http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8113171
http://purl.uniprot.org/citations/8113171http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8113171