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http://purl.uniprot.org/citations/8119278http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8119278http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8119278http://www.w3.org/2000/01/rdf-schema#comment"The periplasmic nitrate reductase of Thiosphaera pantotropha has been purified from a mutant strain (M-6) that overproduces the enzyme activity under anaerobic growth conditions. The enzyme is a complex of a 93-kDa polypeptide and a 16-kDa nitrate-oxidizable cytochrome c552. The complex contains molybdenum; a fluorescent compound with spectral features of a pterin derivative can be extracted. In contrast to the dissimilatory membrane-bound nitrate reductases, the periplasmic nitrate reductase shows high specificity for nitrate as a substrate and is insensitive to inhibition by azide. The 93-kDa subunit exhibits immunological cross-reactivity with the catalytic subunit of Rhodobacter capsulatus N22DNAR+ periplasmic nitrate reductase. Mass spectrometric comparisons of holo-cytochrome c552 and apo-cytochrome c552 demonstrated that the polypeptide bound two haem groups. Mediated redox potentiometry of the cytochrome indicated that the haem groups have reduction potentials (pH = 7.0) of approximately -15 mV and + 80 mV. The functional significance of these potentials is discussed in relation to the proposed physiological role of the enzyme as a redox valve."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1994.tb18605.x"xsd:string
http://purl.uniprot.org/citations/8119278http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1994.tb18605.x"xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Ferguson S.J."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Ferguson S.J."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Robinson C."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Robinson C."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Richardson D.J."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Richardson D.J."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Aplin R.T."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Aplin R.T."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Berks B.C."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Berks B.C."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Reilly A."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/author"Reilly A."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/pages"117-124"xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/pages"117-124"xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/title"Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha."xsd:string
http://purl.uniprot.org/citations/8119278http://purl.uniprot.org/core/title"Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha."xsd:string