| http://purl.uniprot.org/citations/8132663 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8132663 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8132663 | http://www.w3.org/2000/01/rdf-schema#comment | "The short half-life of c-myc mRNA is influenced by sequences in the 3'-untranslated region and the C-terminal part of the coding region. In cell-free extracts, a polysomal protein binds to RNA corresponding to the coding region stability determinant. This and other observations suggest that the protein is bound to polysome-associated c-myc mRNA and protects the mRNA from a ribosome-associated endoribonuclease (Bernstein, P.L., Herrick, D.J., Prokipcak, R.D., and Ross, J. (1992) Genes & Dev. 6, 642-654). Here, we describe a four-step purification of the binding protein: solubilization from ribosomes, ammonium sulfate precipitation, RNA affinity chromatography, and reverse-phase high performance liquid chromatography. The 70-kDa protein can be renatured from solutions containing sodium dodecyl sulfate or organic solvents, greatly facilitating its purification. Protein binding to c-myc coding region RNA is blocked by diamide and N-ethylmaleimide, indicating a requirement for sulfhydryl groups. The protein also binds to N-myc coding region RNA but with approximately 5-fold lower affinity than to the comparable c-myc region. Excess c-myc competitor RNA induces 8-fold destabilization of c-myc mRNA in cell-free mRNA decay extracts. In contrast, N-myc coding region competitor RNA has no effect on c-myc mRNA half-life. Therefore, the protein we have purified probably affects c-myc mRNA metabolism with high specificity."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)37102-8"xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)37102-8"xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/author | "Ross J."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/author | "Ross J."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/author | "Herrick D.J."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/author | "Herrick D.J."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/author | "Prokipcak R.D."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/author | "Prokipcak R.D."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/pages | "9261-9269"xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/pages | "9261-9269"xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/title | "Purification and properties of a protein that binds to the C-terminal coding region of human c-myc mRNA."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/title | "Purification and properties of a protein that binds to the C-terminal coding region of human c-myc mRNA."xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/8132663 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8132663 |
| http://purl.uniprot.org/citations/8132663 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8132663 |
| http://purl.uniprot.org/citations/8132663 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8132663 |
| http://purl.uniprot.org/citations/8132663 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8132663 |