http://purl.uniprot.org/citations/8134375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8134375 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/8134375 | http://www.w3.org/2000/01/rdf-schema#comment | "We report the cloning, characterization, and overexpression of Saccharomyces cerevisiae ERG7, which encodes lanosterol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), EC 5.4.99.7], the enzyme responsible for the complex cyclization/rearrangement step in sterol biosynthesis. Oligonucleotide primers were designed corresponding to protein sequences conserved between Candida albicans ERG7 and the related Arabidopsis thaliana cycloartenol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, cycloartenol forming), EC 5.4.99.8]. A PCR product was amplified from yeast genomic DNA using these primers and was used to probe yeast libraries by hybridization. Partial-length clones homologous to the two known epoxysqualene mutases were isolated, but a full-length sequence was found neither in cDNA nor genomic libraries, whether in phage or plasmids. Two overlapping clones were assembled to make a functional reconstruction of the gene, which contains a 2196-bp open reading frame capable of encoding an 83-kDa protein. The reconstruction complemented the erg7 mutation when driven from either its native promoter or the strong ADH1 promoter."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.91.6.2211"xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.91.6.2211"xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/author | "Bartel B."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/author | "Bartel B."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/author | "Matsuda S.P.T."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/author | "Matsuda S.P.T."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/author | "Corey E.J."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/author | "Corey E.J."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/pages | "2211-2215"xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/pages | "2211-2215"xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/title | "Molecular cloning, characterization, and overexpression of ERG7, the Saccharomyces cerevisiae gene encoding lanosterol synthase."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/title | "Molecular cloning, characterization, and overexpression of ERG7, the Saccharomyces cerevisiae gene encoding lanosterol synthase."xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/volume | "91"xsd:string |
http://purl.uniprot.org/citations/8134375 | http://purl.uniprot.org/core/volume | "91"xsd:string |
http://purl.uniprot.org/citations/8134375 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8134375 |
http://purl.uniprot.org/citations/8134375 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8134375 |
http://purl.uniprot.org/citations/8134375 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8134375 |
http://purl.uniprot.org/citations/8134375 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8134375 |