| http://purl.uniprot.org/citations/8135729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8135729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8135729 | http://www.w3.org/2000/01/rdf-schema#comment | "Prostaglandins inhibit platelet activation by stimulating intracellular cyclic AMP formation. We have postulated that intracellular cyclic AMP levels in platelets are buffered by a distinct prostaglandin receptor that mediates inhibition of cyclic AMP formation. In order to provide evidence for the model, we have cloned the cDNA coding for a prostaglandin receptor EP3 subtype, which is coupled to inhibition of adenylate cyclase, from the megakaryocytic cell line human erythroleukaemia (HEL) cells. A PCR-generated hybridization probe, produced using primers based on the sequence of the mouse prostaglandin EP3 receptor published by Sugimoto, Namba, Honda, Hayashi, Negishi, Ichikawa and Narumiya [(1992) J. Biol. Chem. 267, 6463-6466], was used to screen a lambda gt11 HEL cell cDNA library. The composite full-length cDNA clone HEP3, generated from the two partial clones pHEP3-7 and pHEP3-5, is 1.6 kb long with an open reading frame coding for 390 amino acids. This clone is 83% identical to the alpha subtype of the mouse EP3 receptor. The full-length construct was transfected into COS-1 cells. The cloned receptor exhibited the properties of a prostaglandin EP3 subtype, inhibiting forskolin-stimulated cyclic AMP formation in response to prostaglandin E2 (PGE2) and binding PGE2 with high specificity and a Kd of 3.2 nM. Radiolabelled PGE2 could be displaced by prostaglandins in the order PGE2 = PGE1 > iloprost = PGD2. Northern blot analysis revealed that the receptor is also present in human kidney."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2980263"xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2980263"xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Li S."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Li S."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Bastepe M."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Bastepe M."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Kunapuli S.P."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Kunapuli S.P."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Liu-Chen L.-Y."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Liu-Chen L.-Y."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Ashby B."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Ashby B."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Cheung P.P."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Cheung P.P."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "DeRiel J.K."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "DeRiel J.K."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Fen Mao G."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/author | "Fen Mao G."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8135729 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |