| http://purl.uniprot.org/citations/8138923 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8138923 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8138923 | http://www.w3.org/2000/01/rdf-schema#comment | "Exposure of HeLa cells stably expressing cloned human 5-hydroxytryptamine (5-HT)1A receptors (HA7 cells) to the agonist 8-hydroxy-2-(di-N-propylamino)-tetralin (8-OH-DPAT) results in a loss of high-affinity binding sites and a desensitization of receptor-adenylate cyclase coupling, as measured by 5-HT1A-mediated inhibition of forskolin-stimulated adenylate cyclase activity. These responses can also be observed after exposure to forskolin, which activates cyclic AMP-dependent protein kinase A or after treatment with known activators of protein kinase C (PKC) such as phorbol-12-myristate 13-acetate (PMA). The responses elicited by exposure to 8-OH-DPAT or PMA can be blocked completely by inhibitors of PKC and also by 24-hr exposure to PMA. Preincubation of HA7 cells with 8-OH-DPAT also stimulates hydrolysis of inositol phospholipids and the production of arachidonic acid. Inhibition of phospholipase A2 with quinacrine or by removal of extracellular Ca++ blocks the agonist-mediated loss of 5-HT1A receptor binding sites. These data demonstrate that agonist-induced down regulation of the 5-HT1A receptor occurs after stimulation of both the PKC and phospholipase A2 signaling pathways, both of which may activate PKC. The subsequent response is a loss of high-affinity ligand binding sites and functional receptor coupling to adenylate cyclase."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Ciaranello R.D."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Ciaranello R.D."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Harrington M.A."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Harrington M.A."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Shaw K."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Shaw K."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Zhong P."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/author | "Zhong P."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/name | "J. Pharmacol. Exp. Ther."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/name | "J. Pharmacol. Exp. Ther."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/pages | "1098-1106"xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/pages | "1098-1106"xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/title | "Agonist-induced desensitization and loss of high-affinity binding sites of stably expressed human 5-HT1A receptors."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/title | "Agonist-induced desensitization and loss of high-affinity binding sites of stably expressed human 5-HT1A receptors."xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/volume | "268"xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://purl.uniprot.org/core/volume | "268"xsd:string |
| http://purl.uniprot.org/citations/8138923 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8138923 |
| http://purl.uniprot.org/citations/8138923 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8138923 |
| http://purl.uniprot.org/citations/8138923 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8138923 |
| http://purl.uniprot.org/citations/8138923 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8138923 |