| http://purl.uniprot.org/citations/8142465 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8142465 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8142465 | http://www.w3.org/2000/01/rdf-schema#comment | "Selenoprotein P was partially purified (> 1000-fold) from human plasma in four chromatographic steps using 75Se-labeled selenoprotein P secreted by HepG2 cells in culture as a marker. The purified preparation was injected into mice and monoclonal antibodies, which precipitated the labeled protein, were generated. Neither of two different monoclonal antibodies had cross-reactivity with plasma from five animal species. Antibodies were coupled to agarose, and selenoprotein P was purified from human plasma by immunoaffinity chromatography followed by chromatography on heparin agarose. With two different matrix-bound monoclonal antibodies, the purification procedure gave two bands on SDS-PAGE with mobilities corresponding to 61 and 55 kDa. Both bands stained for carbohydrate and showed increased electrophoretic mobility after enzymatic deglycosylation. Immunoaffinity chromatography removed approx. one-third of the selenium from plasma or 0.4 mumol Se/l at a total selenium concentration of 1.1 mumol/l, indicating that selenoprotein P constituted this proportion of total plasma selenium in healthy US blood donors."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.org/dc/terms/identifier | "doi:10.1016/0167-4838(94)90014-0"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.org/dc/terms/identifier | "doi:10.1016/0167-4838(94)90014-0"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/author | "Burk R.F."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/author | "Burk R.F."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/author | "Aakesson B."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/author | "Aakesson B."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/author | "Bellew T."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/author | "Bellew T."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/pages | "243-249"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/pages | "243-249"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/title | "Purification of selenoprotein P from human plasma."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/title | "Purification of selenoprotein P from human plasma."xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/volume | "1204"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://purl.uniprot.org/core/volume | "1204"xsd:string |
| http://purl.uniprot.org/citations/8142465 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8142465 |
| http://purl.uniprot.org/citations/8142465 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8142465 |
| http://purl.uniprot.org/citations/8142465 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8142465 |
| http://purl.uniprot.org/citations/8142465 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8142465 |