| http://purl.uniprot.org/citations/8144501 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8144501 | http://www.w3.org/2000/01/rdf-schema#comment | "Ferredoxin isolated from the cyanobacterium Synechocystis sp. PCC 6803 has been chemically cross-linked to purified photosystem I from the same organism. The reaction was catalyzed by N-ethyl-3-(3-dimethylaminopropyl)carbodiimide in the presence of N-hydroxysulfosuccinimide. A short reaction time and neutral pH values can be used in the presence of the two reagents, ensuring the integrity of both of the proteins and the iron-sulfur cluster of the ferredoxin. The only covalent complex detected comprised ferredoxin and the photo-system I (PSI)-D subunit, as identified by antibodies probing after electrophoresis. Electron paramagnetic resonance measurements of this covalent complex have shown that the cross-linked ferredoxin was entirely photoreducible by photosystem I and that the molar ratio of ferredoxin to PSI was close to 1. Extensive sequencing of the peptides obtained after proteolysis of the purified cross-linked product led to the identification of a covalent bond between glutamic acid 93 of ferredoxin and lysine 106 of the PSI-D subunit."xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)36986-7"xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/author | "Lelong C."xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/author | "Bottin H."xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/author | "Lagoutte B."xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/author | "Setif P."xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/pages | "10034-10039"xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/title | "Identification of the amino acids involved in the functional interaction between photosystem I and ferredoxin from Synechocystis sp. PCC 6803 by chemical cross-linking."xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/8144501 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8144501 |
| http://purl.uniprot.org/citations/8144501 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8144501 |
| http://purl.uniprot.org/uniprot/#_P27320-mappedCitation-8144501 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8144501 |
| http://purl.uniprot.org/uniprot/#_P19569-mappedCitation-8144501 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8144501 |
| http://purl.uniprot.org/uniprot/P19569 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8144501 |
| http://purl.uniprot.org/uniprot/P27320 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8144501 |