| http://purl.uniprot.org/citations/8144603 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8144603 | http://www.w3.org/2000/01/rdf-schema#comment | "Bovine chromaffin cells are nondividing primary secretory cells that store and secrete catecholamine and a variety of proteins including chromogranins, opiate peptides, and opiate precursors. A transient transfection technique based upon the expression of human growth hormone as a reporter for the regulated secretory pathway was used to study the role of a Ras-like, GTP-binding protein, Rab3a, in Ca(2+)-dependent exocytosis. Immunocytochemistry and flow cytometry revealed that growth hormone and Rab proteins were coexpressed in the same cells. Overexpression of the wild type protein and expression of a mutant protein Rab3aQ81L both inhibited nicotinic agonist-stimulated exocytosis in intact cells. Expression of Rab3aQ81L also inhibited Ca(2+)-dependent secretion from permeabilized cells. Two other mutants, Rab3aN135I and Rab3aT36N, which correspond to dominant acting mutants of Ras, caused limited and no inhibition, respectively, of agonist-stimulated exocytosis. These data provide direct evidence that Rab3a plays an important role in Ca(2+)-triggered exocytosis. We suggest that Rab3a is an inhibitor of secretion, perhaps as part of a pre-fusion complex with secretory vesicles. Elevated Ca2+ may trigger exocytosis by overcoming the inhibition by Rab3a."xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)34051-6"xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/author | "Holz R.W."xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/author | "Macara I.G."xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/author | "Brondyk W.H."xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/author | "Kuizon L."xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/author | "Senter R.A."xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/pages | "10229-10234"xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/title | "Evidence for the involvement of Rab3A in Ca(2+)-dependent exocytosis from adrenal chromaffin cells."xsd:string |
| http://purl.uniprot.org/citations/8144603 | http://purl.uniprot.org/core/volume | "269"xsd:string |
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