| http://purl.uniprot.org/citations/8174709 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8174709 | http://www.w3.org/2000/01/rdf-schema#comment | "A mutant of spinach ferredoxin-NADP+ reductase, in which Lys-88 has been changed to glutamine, has been obtained by site-directed mutagenesis. The mutant enzyme was fully active as a diaphorase, but partially impaired in ferredoxin-dependent cytochrome c reductase activity. By steady-state kinetics, the Km for ferredoxin of the K88Q enzyme was found to have increased 10-fold, whereas the kcat was unaffected by the amino acid replacement. The interaction between oxidized ferredoxin and the enzyme forms was also studied by spectrofluorimetric titration: Kd values of 110 and 10 nM were determined for the mutant and wild-type proteins, respectively. These data point out the importance of a positive charge at position 88 of the reductase for the interaction with ferredoxin, confirming previous cross-linking studies."xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(94)80565-2"xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/author | "Aliverti A."xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/author | "Zanetti G."xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/author | "Corrado M.E."xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/name | "FEBS Lett"xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/pages | "247-250"xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/title | "Involvement of lysine-88 of spinach ferredoxin-NADP+ reductase in the interaction with ferredoxin."xsd:string |
| http://purl.uniprot.org/citations/8174709 | http://purl.uniprot.org/core/volume | "343"xsd:string |
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| http://purl.uniprot.org/uniprot/#_P00221-mappedCitation-8174709 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8174709 |
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