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http://purl.uniprot.org/citations/8175719http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8175719http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8175719http://www.w3.org/2000/01/rdf-schema#comment"HB-GAM (heparin binding growth-associated molecule; pleiotrophin) is a secretory, extracellular matrix-associated protein that is strongly expressed in developing nervous tissues and belongs to a novel family of differentiation/growth factors. It promotes axonal growth from perinatal rat brain neurons and is suggested to be mitogenic for some cell types and to display cell-transforming activity. Since the receptors of HB-GAM in cells are unknown, we have started isolation of putative cell surface receptors from brain neurons and from perinatal rat brain. For this purpose, recombinant HB-GAM was produced with the aid of a baculovirus vector and used as an affinity matrix in receptor isolation. A detergent-solubilized component from cultured brain neurons and from brain was identified that binds specifically to HB-GAM and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a broad smear with an apparent molecular mass of about 200 kDa. This cell surface component was found to contain heparan sulfate chains, which are bound to a core protein with an apparent molecular mass of 120 kDa. Gel electrophoretic characteristics, immunochemical analysis, and partial peptide sequencing revealed that the cell surface component isolated as an HB-GAM receptor is N-syndecan (syndecan-3). In a solid phase binding assay, N-syndecan was found to bind to HB-GAM in a similar manner as to basic fibroblast growth factor (KD = 0.6 nM). Immunofluorescence microscopy indicated that in brain neurons, N-syndecan occurs at the surface of the cell soma and of the neurites that grow along HB-GAM-coated substrates. Anti-N-syndecan antibodies added to culture media had an inhibitory effect on HB-GAM-induced neurite outgrowth. We suggest that N-syndecan mediates the neurite outgrowth-promoting signal from HB-GAM to the cytoskeleton of growing neurites."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)99975-8"xsd:string
http://purl.uniprot.org/citations/8175719http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)99975-8"xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Carey D.J."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Carey D.J."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Raulo E."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Raulo E."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Rauvala H."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Rauvala H."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Chernousov M.A."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Chernousov M.A."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Nolo R."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/author"Nolo R."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/date"1994"xsd:gYear
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/pages"12999-13004"xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/pages"12999-13004"xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/title"Isolation of a neuronal cell surface receptor of heparin binding growth-associated molecule (HB-GAM). Identification as N-syndecan (syndecan-3)."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/title"Isolation of a neuronal cell surface receptor of heparin binding growth-associated molecule (HB-GAM). Identification as N-syndecan (syndecan-3)."xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/volume"269"xsd:string
http://purl.uniprot.org/citations/8175719http://purl.uniprot.org/core/volume"269"xsd:string
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