| http://purl.uniprot.org/citations/8195171 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8195171 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8195171 | http://www.w3.org/2000/01/rdf-schema#comment | "The Src homology 2 (SH2) domain-containing Shc proteins p52shc and p46shc become phosphorylated upon activation of several tyrosine kinases and are implicated in mitogenic signal transduction. Ligand stimulation of the platelet-derived growth factor (PDGF) beta-receptor leads to autophosphorylation of tyrosine residues, which is known to mediate interactions with several SH2 domain-containing signaling molecules. In this study, we have characterized the interaction between the PDGF beta-receptor and Shc. PDGF beta-receptor coprecipitation in Shc immunoprecipitates was dependent on stimulation with PDGF-BB. The Shc SH2 domain expressed as a bacterial fusion protein bound the autophosphorylated PDGF beta-receptor. Moreover, the Shc SH2 domain could bind the autophosphorylated purified baculovirus-expressed PDGF beta-receptor intracellular domain, which indicates a direct association of Shc with the PDGF beta-receptor. Activation of the PDGF beta-receptor induced the preferential phosphorylation of p52shc. Tyrosine-phosphorylated Shc, in turn, formed a complex with the signaling molecule Grb2. Synthetic peptide analysis revealed that certain autophosphorylation sites in the PDGF beta-receptor (Tyr-579, Tyr-740, Tyr-751, and Tyr-771) were able to mediate the specific binding of the Shc SH2 domain as well as intact Shc proteins. A mutant PDGF beta-receptor in which Tyr-579 was replaced with phenylalanine showed 40% impaired association of Shc in vivo, but phosphorylation of Shc proteins was not affected. We conclude that multiple autophosphorylation sites in the PDGF beta-receptor are responsible for the binding of Shc. This is in contrast to previously characterized interactions between the PDGF beta-receptor and SH2 domain-containing proteins, which generally involve one high affinity binding site in the receptor."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)36611-5"xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)36611-5"xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Hansen K."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Hansen K."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Mori S."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Mori S."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Pawson T."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Pawson T."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Heldin C.H."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Heldin C.H."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Claesson-Welsh L."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Claesson-Welsh L."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Yokote K."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "Yokote K."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "McGlade J."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/author | "McGlade J."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/pages | "15337-15343"xsd:string |
| http://purl.uniprot.org/citations/8195171 | http://purl.uniprot.org/core/pages | "15337-15343"xsd:string |