| http://purl.uniprot.org/citations/8195297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8195297 | http://www.w3.org/2000/01/rdf-schema#comment | "A typical mammalian egg is surrounded by an outer layer of about 3,000 cumulus cells embedded in an extracellular matrix rich in hyaluronic acid. A current, widely proposed model is that the fertilizing sperm, while it is acrosome intact, passes through the cumulus cell layer and binds to the egg zona pellucida. This current model lacks a well-supported explanation for how sperm penetrate the cumulus layer. We report that the sperm protein PH-20 has a hyaluronidase activity and is present on the plasma membrane of mouse and human sperm. Brief treatment with purified, recombinant PH-20 can release all the cumulus cells surrounding mouse eggs. Acrosome intact mouse sperm incubated with anti-PH-20 antibodies can not pass through the cumulus layer and thus can not reach the zona pellucida. These results, indicating that PH-20 enables acrosome intact sperm to penetrate the cumulus barrier, reveal a mechanism for cumulus penetration, and thus provide the missing element in the current model."xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.125.5.1157"xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/author | "Lin Y."xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/author | "Myles D.G."xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/author | "Primakoff P."xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/author | "Lathrop W.F."xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/author | "Mahan K."xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/pages | "1157-1163"xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/title | "A hyaluronidase activity of the sperm plasma membrane protein PH-20 enables sperm to penetrate the cumulus cell layer surrounding the egg."xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://purl.uniprot.org/core/volume | "125"xsd:string |
| http://purl.uniprot.org/citations/8195297 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8195297 |
| http://purl.uniprot.org/citations/8195297 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8195297 |
| http://purl.uniprot.org/uniprot/P38567#attribution-D42A5A41D2B9F649044D8A2F814A04B1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8195297 |
| http://purl.uniprot.org/uniprot/#_P38567-mappedCitation-8195297 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8195297 |
| http://purl.uniprot.org/uniprot/P38567 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8195297 |