| http://purl.uniprot.org/citations/8196478 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8196478 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8196478 | http://www.w3.org/2000/01/rdf-schema#comment | "alpha 1-Adrenoceptors (ARs) play an important role in mediating human prostatic smooth muscle contraction. In the present study cDNA fragments covering different domains of 3 alpha 1-AR subtypes (alpha 1b, alpha 1c and alpha 1d) were generated from human prostate by reverse transcription coupled to the polymerase chain reaction (RT-PCR). The reconstituted partial sequence (349 amino acids) of the human prostatic alpha 1c-AR PCR products showed 94% identity at the amino acid level with that of the corresponding region of the cloned bovine brain alpha 1c-AR. Using human alpha 1-AR subtype selective cDNA probes in Northern blot analysis, the co-expression of mRNA transcripts corresponding to alpha 1b-, alpha 1c- and alpha 1d-AR subtypes was detected in 4 different regions (apex, base, periurethra and lateral lobe) of the human prostate. Competitive inhibition experiments of [3H]-prazosin binding to membrane preparations of human prostate revealed that the non-selective alpha 1-subtype antagonist, alfuzosin, produced a monophasic inhibition curve, whereas oxymetazoline produced a 2-component inhibition curve with pKi values of 8.54 and 5.46. The high-affinity alpha 1-AR component of the oxymetazoline inhibition curve was predominant (57%-66%) and showed an affinity for oxymetazoline comparable to that of the alpha 1c-AR subtype. As such our results illustrate the expression of different alpha 1-AR subtypes in human prostate and importantly that alpha 1c represents the predominant alpha 1-AR subtype present in this tissue."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.org/dc/terms/identifier | "doi:10.1016/0024-3205(94)90031-0"xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.org/dc/terms/identifier | "doi:10.1016/0024-3205(94)90031-0"xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Faure C."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Faure C."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Graham D."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Graham D."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Langer S.Z."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Langer S.Z."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Pimoule C."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Pimoule C."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Vallancien G."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/author | "Vallancien G."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/name | "Life Sci."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/name | "Life Sci."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/pages | "1595-1605"xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/pages | "1595-1605"xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/title | "Identification of alpha 1-adrenoceptor subtypes present in the human prostate."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/title | "Identification of alpha 1-adrenoceptor subtypes present in the human prostate."xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/volume | "54"xsd:string |
| http://purl.uniprot.org/citations/8196478 | http://purl.uniprot.org/core/volume | "54"xsd:string |