| http://purl.uniprot.org/citations/8199264 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8199264 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8199264 | http://www.w3.org/2000/01/rdf-schema#comment | "Embryogenesis-stimulating activity (ESA) was found in serum-free conditioned media (CM) of bovine cumulus/granulosa cells (BGC) and bovine oviductal epithelial cells (BOEC). The CM of BGC (BGC-CM) contained two molecular species of ESA, one with a low molecular weight (M(r) 30,000) and another with a high molecular weight (M(r) 80,000); but only the activity with low molecular weight was detected in CM of BOEC by gel-permeation chromatography. The smaller ESA (embryogenin-1) in BGC-CM was purified to homogeneity, as a common activity in both CM by a combination of gel-permeation chromatography, ion-exchange chromatography, and reverse-phase HPLC. Embryogenin-1 has a molecular weight of 31,100 (reduced) and has been identified as a bovine tissue inhibitor of metalloproteinase-1 by NH2-terminal amino acid sequence analysis. Western blot analysis, anti-proteinase activities against metalloproteinases, and the nucleotide sequence of cDNA isolated from a lambda gt11 cDNA library of the bovine ovary by a polyclonal antibody against embryogenin-1. These data suggest that the tissue inhibitor of metalloproteinase-1 produced by BGC and BOEC is a major ESA for in vitro development of bovine embryos."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod50.4.835"xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod50.4.835"xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Satoh T."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Satoh T."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Yamashita S."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Yamashita S."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Kikuchi M."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Kikuchi M."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Sendai Y."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Sendai Y."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Hoshi H."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/author | "Hoshi H."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/name | "Biol. Reprod."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/name | "Biol. Reprod."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/pages | "835-844"xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/pages | "835-844"xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/title | "Tissue inhibitor of metalloproteinases (TIMP-1) produced by granulosa and oviduct cells enhances in vitro development of bovine embryo."xsd:string |
| http://purl.uniprot.org/citations/8199264 | http://purl.uniprot.org/core/title | "Tissue inhibitor of metalloproteinases (TIMP-1) produced by granulosa and oviduct cells enhances in vitro development of bovine embryo."xsd:string |