| http://purl.uniprot.org/citations/8231828 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8231828 | http://www.w3.org/2000/01/rdf-schema#comment | "The effect of an increased intracellular dCTP:dTTP ratio on the specificities of ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) mutagenesis was examined in the yeast Saccharomyces cerevisiae. To do so, we used a dCMP deaminase-deficient (dcd1) strain having a dCTP:dTTP ratio > 77-fold larger than its isogenic wild-type parent under the treatment conditions employed. This DNA precursor imbalance lowered the frequencies of EMS- or MNNG-induced SUP4-o mutations by 75 or 45%, respectively, relative to the corresponding values for the wild-type strain. A total of 405 SUP4-o mutations produced by the alkylating agents in the dcd1 background were characterized by DNA sequencing and the mutational spectra were compared to those for 399 mutations induced in the wild-type parent and 207 mutations that arose spontaneously in the dcd1 strain. Unexpectedly, the frequencies of EMS- and MNNG-induced GC-->AT transitions in the dcd1 strain were found to be reduced by 93 and 68%, respectively, considerably more than the decreases for the overall SUP4-o mutation frequencies. The differences were due mainly to substantial increases in the frequencies of GC-->CG transversions. Although these events were the predominant type of spontaneous substitution in the dcd1 strain, they were more frequent after alkylation treatment and were distributed differently than the spontaneous GC-->CG transversions. Preferences for the EMS- or MNNG-induced GC-->AT transitions to occur at GC sites having the guanine located on the transcribed strand or preceded by a 5' purine, respectively, also were diminished in the dcd1 strain.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.org/dc/terms/identifier | "doi:10.1093/mutage/8.5.457"xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/author | "Kohalmi S.E."xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/author | "Kunz B.A."xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/author | "Roche H.M."xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/name | "Mutagenesis"xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/pages | "457-465"xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/title | "Elevated intracellular dCTP levels reduce the induction of GC-->AT transitions in yeast by ethyl methanesulfonate or N-methyl-N'-nitro-N- nitrosoguanidine but increase alkylation-induced GC-->CG transversions."xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/8231828 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8231828 |
| http://purl.uniprot.org/citations/8231828 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8231828 |
| http://purl.uniprot.org/uniprot/#_P06773-mappedCitation-8231828 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8231828 |
| http://purl.uniprot.org/uniprot/P06773 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/8231828 |