| http://purl.uniprot.org/citations/8280099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8280099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8280099 | http://www.w3.org/2000/01/rdf-schema#comment | "Tetrahymena pyriformis was found to exhibit high NADPH-dependent 20-oxosteroid reductase activity that converted 17 alpha-hydroxyprogesterone into 17 alpha,20 alpha-dihydroxypregn-4-en-3-one. The enzyme was purified 400-fold from the cytosolic fraction. The purified enzyme with a specific activity of 6.4 mumol/min per mg of protein had an isoelectric point of 4.9 and M(r) of 68,000, and was composed of two subunits of equal size. The N-terminal sequence was determined to be LAKTVPLNDGTNFPIFGG. The enzyme reduced pregnanes and pregnanes possessing a 17 alpha-hydroxy group to a greater extent than those without the hydroxy group, and oxidized 20 alpha-hydroxy groups of the steroids in the presence of NADP+. The Km values for 17 alpha-hydroxyprogesterone and 17 alpha-hydroxypregnenolone were 2.9 and 3.4 microM respectively. Although the enzyme was inactive towards androgens and oestrogens with 3- or 17-oxo groups, it reduced several nonsteroidal carbonyl compounds and oxidized trans-benzene dihydrodiol. The enzyme activity was inhibited by synthetic oestrogens, barbiturates, aldose reductase inhibitors and quercitrin. Thus, this enzyme is a novel form of 20 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.149) which structurally and functionally differs from the mammalian and bacterial enzymes."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2970195"xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2970195"xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Sato K."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Sato K."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Nozawa Y."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Nozawa Y."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Hara A."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Hara A."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Inazu A."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Inazu A."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Nakayama Y."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/author | "Nakayama Y."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/pages | "195-200"xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/pages | "195-200"xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/title | "Purification and characterization of a novel dimeric 20 alpha-hydroxysteroid dehydrogenase from Tetrahymena pyriformis."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/title | "Purification and characterization of a novel dimeric 20 alpha-hydroxysteroid dehydrogenase from Tetrahymena pyriformis."xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/volume | "297"xsd:string |
| http://purl.uniprot.org/citations/8280099 | http://purl.uniprot.org/core/volume | "297"xsd:string |