Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/8293998http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8293998http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8293998http://www.w3.org/2000/01/rdf-schema#comment"The murine genes, gamma B-cry and gamma C-cry, encoding the gamma B- and gamma C-crystallins, were isolated from a genomic DNA library. The complete nucleotide (nt) sequences of both genes were determined from 661 and 711 bp, respectively, upstream from the first exon to the corresponding polyadenylation sites, comprising more than 2650 and 2890 bp, respectively. The new sequences were compared to the partial cDNA sequences available for the murine gamma B-cry and gamma C-cry, as well as to the corresponding genomic sequences from rat and man, at both the nt and predicted amino acid (aa) sequence levels. In the gamma B-cry promoter region, a canonical CCAAT-box, a TATA-box, putative NF-I and C/EBP sites were detected. An R-repeat is inserted 366 bp upstream from the transcription start point. In contrast, the gamma C-cry promoter does not contain a CCAAT-box, but some other putative binding sites for transcription factors (AP-2, UBP-1, LBP-1) were located by computer analysis. The promoter regions of all six gamma-cry from mouse, rat and human, except human psi gamma F-cry, were analyzed for common sequence elements. A complex sequence element of about 70-80 bp was found in the proximal promoter, which contains a gamma-cry-specific and almost invariant sequence (crygpel) of 14 nt, and ends with the also invariant TATA-box. Within the complex sequence element, a minimum of three further features specific for the gamma A-, gamma B- and gamma D/E/F-cry genes can be defined, at least two of which were recently shown to be functional. In addition to these four sequence elements, a subtype-specific structure of inverted repeats with different-sized spacers can be deduced from the multiple sequence alignment. A phylogenetic analysis based on the promoter region, as well as the complete exon 3 of all gamma-cry from mouse, rat and man, suggests separation of only five gamma-cry subtypes (gamma A-, gamma B-, gamma C-, gamma D- and gamma E/F-cry) prior to species separation."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(93)90458-f"xsd:string
http://purl.uniprot.org/citations/8293998http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(93)90458-f"xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Werner T."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Werner T."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Graw J."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Graw J."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Liebstein A."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Liebstein A."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Pietrowski D."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Pietrowski D."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Schmitt-John T."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/author"Schmitt-John T."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/pages"145-156"xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/pages"145-156"xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/title"Genomic sequences of murine gamma B- and gamma C-crystallin-encoding genes: promoter analysis and complete evolutionary pattern of mouse, rat and human gamma-crystallins."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/title"Genomic sequences of murine gamma B- and gamma C-crystallin-encoding genes: promoter analysis and complete evolutionary pattern of mouse, rat and human gamma-crystallins."xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/volume"136"xsd:string
http://purl.uniprot.org/citations/8293998http://purl.uniprot.org/core/volume"136"xsd:string
▶