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http://purl.uniprot.org/citations/8294010http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8294010http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8294010http://www.w3.org/2000/01/rdf-schema#comment"The gene (aprII) encoding alkaline serine protease (AprII; subtilase) from Alteromonas sp. strain O-7 was cloned in plasmid pUC19 and transformed into Escherichia coli JM109. The nucleotide (nt) sequence of aprII has been determined. A single open reading frame (ORF) encoded a protein consisting of 621 amino acids (aa) with a M(r) of 63,958. The results of aa sequence analysis indicated that AprII is produced as a large precursor consisting of four domains: the signal sequence, the N-terminal pro-region (AprII-N), the mature AprII (AprII-M) and the C-terminal pro-region (AprII-C). The aa sequence of AprII-M shows high sequence homology with those of class-II subtilases. Two conserved sequences were found in AprII-N which might play a critical role in the maintenance of chaperone-like activity. Repeated aa sequences were observed in AprII-C (AprII-C1 and AprII-C2). The aa sequences of AprII-C1 and AprII-C2 show high sequence homology with those of the C-terminal pro-region of the other known proteases."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(93)90473-G"xsd:string
http://purl.uniprot.org/citations/8294010http://purl.org/dc/terms/identifier"doi:10.1016/0378-1119(93)90473-g"xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Tanaka K."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Tanaka K."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Imada C."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Imada C."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Miyamoto K."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Miyamoto K."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Kawai M."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Kawai M."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Inamori Y."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Inamori Y."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Okami Y."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Okami Y."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Tsujibo H."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Tsujibo H."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Tainaka K."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/author"Tainaka K."xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/8294010http://purl.uniprot.org/core/name"Gene"xsd:string