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http://purl.uniprot.org/citations/8325890http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8325890http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8325890http://www.w3.org/2000/01/rdf-schema#comment"We have previously reported the presence of a 28-kDa protein in human mammary adenocarcinoma MCF-7 cells, whose phosphorylation by phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) and permeant diacylglycerol 1,2-dioctanoyl-sn-glycerol was correlated to growth arrest induced by the protein kinase C (PKC) activators. We now investigate the possible identity of this protein with the estrogen-regulated "24-kDa" protein shown as related to the mammalian heat shock protein 27 (Fuqua, S. A. W., Blum-Salingaros, M., and McGuire, W. L. (1989) Cancer Res 49, 4126-4129). 32P-Labeled 28-kDa protein from TPA-treated MCF-7 cells was immunoprecipitated with a 24-kDa-specific monoclonal antibody. Immunoblots from cell extracts fractionated by two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis demonstrated that TPA induced the conversion of a 28-kDa isoform "a" (pI 6.7) to a more acidic isoform "b" (pI 6.2). Two-dimensional gel analysis of [3H]leucine-labeled MCF-7 cell extracts demonstrated that conversely to TPA, which induced only phosphorylation of 28-kDa protein, heat shock induced both synthesis (increase of isoform a) and phosphorylation (conversion of isoforms a to b) of the protein. 32P labeling of MCF-7 cells allowed demonstration of the presence of an extra phosphoisoform "c" (pI 5.9) upon TPA as well as heat shock treatment. When cells were pretreated with the bisindolylmaleimide GF109203X, a selective inhibitor of PKC, the heat shock-induced phosphorylation was unchanged, while the TPA effect was almost abolished, suggesting that the heat shock-activated protein kinase was very likely different from PKC. However, peptide mapping of the 28-kDa phosphoprotein suggested identical sites of phosphorylation upon TPA and heat shock stimulation. Partial amino acid sequencing of the 28-kDa protein revealed identity with both the 24-kDa protein and the mammalian HSP27. The fact that estrogens and PKC, respectively, regulate expression and phosphorylation of this 24/28-kDa protein strongly argues for its key role in MCF-7 cell proliferation and differentiation."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)82451-6"xsd:string
http://purl.uniprot.org/citations/8325890http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)82451-6"xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Ferrara P."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Ferrara P."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Capdevielle J."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Capdevielle J."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Mazarguil H."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Mazarguil H."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Canal I."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Canal I."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Darbon J.-M."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Darbon J.-M."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Faucher C."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"Faucher C."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"McGuire W.L."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/author"McGuire W.L."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/pages"15168-15173"xsd:string
http://purl.uniprot.org/citations/8325890http://purl.uniprot.org/core/pages"15168-15173"xsd:string
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