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http://purl.uniprot.org/citations/8331075http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8331075http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8331075http://www.w3.org/2000/01/rdf-schema#comment"The TolQ and TolR proteins of Escherichia coli are required for the uptake of group A colicins and for infection by filamentous phages. Their topology in the cytoplasmic membrane was determined by cleavage with aminopeptidase K, proteinase K, and trypsin in spheroplasts and cell lysates. From the results obtained, it is proposed that the N terminus of TolQ is located in the periplasm and that it contains three transmembrane segments (residues 9 to 36, 127 to 159, and 162 to 191), a small periplasmic loop, and two large portions in the cytoplasm. The N terminus of TolR is located in the cytoplasm and is followed by a transmembrane segment (residues 21 to 40), and the remainder of the protein is located in the periplasm. A tolQ mutant, which rendered cells resistant to group A colicins and sensitive to cholate, had alanine 13 replaced by glycine and was lacking serine 14 in the first transmembrane segment. The membrane topologies of TolQ and TolR are similar to those proposed for ExbB and ExbD, respectively, which is consistent with the partial functional substitution between ExbB and TolQ and between ExbD and TolR. The amino acid sequences of these proteins display the highest homology in the transmembrane segments, which indicates that the membrane-spanning regions play an important role in the activities of the proteins."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.org/dc/terms/identifier"doi:10.1128/jb.175.14.4485-4491.1993"xsd:string
http://purl.uniprot.org/citations/8331075http://purl.org/dc/terms/identifier"doi:10.1128/jb.175.14.4485-4491.1993"xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/author"Braun V."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/author"Braun V."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/author"Kampfenkel K."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/author"Kampfenkel K."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/pages"4485-4491"xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/pages"4485-4491"xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/title"Membrane topologies of the TolQ and TolR proteins of Escherichia coli: inactivation of TolQ by a missense mutation in the proposed first transmembrane segment."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/title"Membrane topologies of the TolQ and TolR proteins of Escherichia coli: inactivation of TolQ by a missense mutation in the proposed first transmembrane segment."xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/volume"175"xsd:string
http://purl.uniprot.org/citations/8331075http://purl.uniprot.org/core/volume"175"xsd:string
http://purl.uniprot.org/citations/8331075http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8331075
http://purl.uniprot.org/citations/8331075http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8331075
http://purl.uniprot.org/citations/8331075http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8331075
http://purl.uniprot.org/citations/8331075http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8331075
http://purl.uniprot.org/uniprot/P0ABV6http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/8331075
http://purl.uniprot.org/uniprot/P0ABU9http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/8331075