| http://purl.uniprot.org/citations/8336731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8336731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8336731 | http://www.w3.org/2000/01/rdf-schema#comment | "n-Chimerin (alpha 1-chimerin) is a brain GTPase-activating protein (GAP) for the ras-related p21rac. We now report the occurrence of another form of chimerin, termed alpha 2-chimerin. This is the product of an alternately spliced transcript of the human n-chimerin gene encoding an N-terminal SH2 (src homology 2) domain in addition to the phorbol ester receptor and GAP domains. alpha 1- and alpha 2-chimerin mRNAs were expressed differently. In the rat brain, only alpha 1-chimerin mRNA was expressed in cerebellar Purkinje cells, although both alpha 1- and alpha 2-chimerin mRNAs occurred in neurons in the cerebral cortex, hippocampus, and thalamus. Only alpha 2-chimerin RNA was expressed in rat testes, in early pachytene spermatocytes. A 45-kDa SH2-containing chimerin corresponding to the alpha 2 form was purified from rat brain. As with Escherichia coli 45-kDa recombinant alpha 2-chimerin, purified brain alpha 2-chimerin exhibited racGAP activity which was stimulated by phosphatidylserine. The recombinant SH2 domain bound several 32P-labelled phosphoproteins of PC12 cells, whose phosphorylation increased in response to trophic factors, including nerve growth factor. To examine the relationships of alpha 1- and alpha 2-chimerin transcripts, human genomic DNA clones were characterized. In alpha 2-chimerin mRNA, a 3' splice acceptor site within exon 1 of alpha 1-chimerin mRNA was used, replacing its 5' untranslated region and N-terminal coding sequence. The single human n-chimerin gene was mapped to chromosome 2q31-q32.1, colocalizing with the CRE-BP1 transcription factor gene (2q32). It contained several splice junctions conserved with the sequence-related protein kinase C and bcr genes. alpha 2-Chimerin is only the second SH2-containing GAP and the first example of an SH2 domain generated by alternate splicing."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.13.8.4986-4998.1993"xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.13.8.4986-4998.1993"xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Lim L."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Lim L."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Hall C."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Hall C."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Jones T.A."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Jones T.A."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Smith P."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Smith P."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Manser E."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Manser E."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Teo M."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Teo M."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Lim H.H."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Lim H.H."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Spurr N.K."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Spurr N.K."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Dong J.M."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Dong J.M."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Michael G.J."xsd:string |
| http://purl.uniprot.org/citations/8336731 | http://purl.uniprot.org/core/author | "Michael G.J."xsd:string |