| http://purl.uniprot.org/citations/8373353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8373353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8373353 | http://www.w3.org/2000/01/rdf-schema#comment | "The 1.54 kb cDNA for ancrod, a thrombin-like enzyme, was cloned from a lambda ZAP cDNA library derived from the venom glands of Calloselasma (Agkistrodon) rhodostoma. The cDNA sequence reveals that ancrod is synthesized as a pre-zymogen of 258 amino acids, including a putative secretory peptide of 18 amino acids and a proposed zymogen peptide of 6 amino-acid residues. The amino-acid sequence of the predicted active form of the enzyme exhibits a high degree of sequence similarity to those of mammalian serine proteases (trypsin and pancreatic kallikrein) and other thrombin-like enzymes (batroxobin and flavoxobin). Key amino-acid residues (His43, Asp88, Ser182 and Asp176) that are thought to be involved in the substrate cleavage and in the substrate-binding reaction are conserved. Ancrod contains 13 cysteine residues. Based on alignment with the amino-acid sequences of trypsin and batroxobin, six disulphide bridges can be predicted to be present in the ancrod protein. The existence of a free cysteine, which changes the common sequence surrounding the Ser182 active site from Gly-Asp-Ser-Gly-Gly-Pro to Cys-Asp-Ser-Gly-Gly-Pro, is unusual for a serine protease."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2940387"xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2940387"xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Shih C.-M."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Shih C.-M."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Au L.-C."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Au L.-C."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Teh G.-W."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Teh G.-W."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Chang K.-J."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Chang K.-J."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Chou J.-S."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Chou J.-S."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Lin S.-B."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/author | "Lin S.-B."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/pages | "387-390"xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/pages | "387-390"xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/title | "Molecular cloning and sequence analysis of the cDNA for ancrod, a thrombin-like enzyme from the venom of Calloselasma rhodostoma."xsd:string |
| http://purl.uniprot.org/citations/8373353 | http://purl.uniprot.org/core/title | "Molecular cloning and sequence analysis of the cDNA for ancrod, a thrombin-like enzyme from the venom of Calloselasma rhodostoma."xsd:string |