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http://purl.uniprot.org/citations/8394362http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8394362http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8394362http://www.w3.org/2000/01/rdf-schema#comment"We have utilized the polymerase chain reaction technique to selectively amplify a G protein-coupled receptor cDNA from rat kidney proximal convoluted tubule mRNA, which exhibits high homology with previously cloned serotonin receptors. Sequencing of a full-length clone isolated from a rat hippocampal cDNA library revealed an open reading frame of 1,212 base pairs encoding a 404-residue protein with seven hydrophobic regions predicted to represent transmembrane-spanning domains. Within the transmembrane regions, this receptor was found to be 44-50% identical with various members of the 5-HT1, 5-HT5, and 5-HT6 subfamilies with lower (37-40%) homology to the 5-HT2-like receptors. Northern blots revealed a approximately 3.6-kilobase transcript localized in various brain regions with the following rank order of abundance: hypothalamus > hippocampus = mesencephalon > cerebral cortex = olfactory bulb > olfactory tubercle. Expression of this clone in COS-7 cells resulted in the appearance of high affinity, saturable binding of [3H]lysergic acid diethylamide ([3H]LSD; KD = 5 nM) and [3H]serotonin ([3H]5-HT; KD = 1 nM). Among endogenous biogenic amines, only 5-HT completely inhibited radioligand binding. The inhibition of radioligand binding by other serotonergic agents revealed a pharmacological profile that does not correlate with any previously described serotonin receptor subtype. In addition, this receptor exhibits high affinity for a number of tricyclic antipsychotic and antidepressant drugs including clozapine, loxapine, and amitriptyline. In HEK-293 cells stably transfected with this receptor, serotonin elicits a potent stimulation of adenylylcyclase activity. The distinct structural and pharmacological properties of this receptor suggests that it represents a completely novel serotonin receptor subtype, which we propose to designate 5-HT7. Based on its pharmacology and its localization to limbic and cortical regions of the brain, it is likely that this receptor may play a role in several neuropsychiatric disorders that involve serotonergic systems."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(17)46830-x"xsd:string
http://purl.uniprot.org/citations/8394362http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(17)46830-x"xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Shen Y."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Shen Y."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Hamblin M.W."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Hamblin M.W."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Metcalf M.A."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Metcalf M.A."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Jose P.A."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Jose P.A."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Monsma F.J. Jr."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Monsma F.J. Jr."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Sibley D.R."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/author"Sibley D.R."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/pages"18200-18204"xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/pages"18200-18204"xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/title"Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype."xsd:string
http://purl.uniprot.org/citations/8394362http://purl.uniprot.org/core/title"Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype."xsd:string
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