| http://purl.uniprot.org/citations/8416914 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8416914 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8416914 | http://www.w3.org/2000/01/rdf-schema#comment | "The anti-sigma 70 factor of bacteriophage T4 is a 10-kDa (10K) protein which inhibits the sigma 70-directed initiation of transcription by Escherichia coli RNA polymerase holoenzyme. We have partially purified the anti-sigma 70 factor and obtained the sequence of a C-terminal peptide of this protein. Using reverse genetics, we have identified, at the end of the lysis gene t and downstream of an as yet unassigned phage T4 early promoter, an open reading frame encoding a 90-amino-acid protein with a predicted molecular weight of 10,590. This protein has been overproduced in a phage T7 expression system and partially purified. It shows a strong inhibitory activity towards sigma 70-directed transcription (by RNA polymerase holoenzyme), whereas it has no significant effect on sigma 70-independent transcription (by RNA polymerase core enzyme). At high ionic strength, this inhibition is fully antagonized by the neutral detergent Triton X-100. Our results corroborate the initial observations on the properties of the phage T4 10K anti-sigma 70 factor, and we therefore propose that the gene which we call asiA, identified in the present study, corresponds to the gene encoding this T4 transcriptional inhibitor."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.175.1.85-93.1993"xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.175.1.85-93.1993"xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Brody E.N."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Brody E.N."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Le Caer J.-P."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Le Caer J.-P."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Orsini G."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Orsini G."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Ouhammouch M."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/author | "Ouhammouch M."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/pages | "85-93"xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/pages | "85-93"xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/title | "The asiA gene of bacteriophage T4 codes for the anti-sigma 70 protein."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/title | "The asiA gene of bacteriophage T4 codes for the anti-sigma 70 protein."xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/volume | "175"xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://purl.uniprot.org/core/volume | "175"xsd:string |
| http://purl.uniprot.org/citations/8416914 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8416914 |
| http://purl.uniprot.org/citations/8416914 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8416914 |