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http://purl.uniprot.org/citations/8417953http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8417953http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8417953http://www.w3.org/2000/01/rdf-schema#comment"The erythroid-potentiating activity (EPA) of the tissue inhibitor of metalloproteinase-1 (TIMP-1) was re-examined using ELM-I-1-3, a mouse erythroleukemia cell line, which responded well to erythropoietin. Depletion of pre-existing TIMP-1 from fetal calf serum in culture medium using monoclonal antibody suppressed erythropoietin-induced differentiation as measured by the induction of hemoglobin, commitment assay and globin mRNAs. The removal of TIMP-1 also suppressed the proliferation of ELM-I-1-3 as measured by cell number and de novo DNA synthesis. These changes were reversed by the addition of purified TIMP-1 to the culture medium. Anti-TIMP-1 antibody also blocked both hexamethylene bisacetamide (HMBA)-induced erythroid differentiation and the proliferation of both ELM-I-1-3 and Friend erythroleukemia cells. Considering previous reports analyzing the chemical induction of Friend mouse erythroleukemia cell differentiation, our results suggest that erythropoietin- or HMBA-induced erythroid differentiation might also be coupled with cell proliferation. Our 3H thymidine-uptake experiment shows that TIMP-1 removal was also effective in the inhibition of cell growth of various other cell lines in addition to erythroleukemia cell lines. These results suggest that EPA action of TIMP-1 on erythroid leukemia cell lines is closely related to its activity to promote the cell growth of various cell lines and cells including erythroleukemia cell lines."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Saito H."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Saito H."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Yoshida S."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Yoshida S."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Kinoshita T."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Kinoshita T."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Yamashita K."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Yamashita K."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Hotta T."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Hotta T."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Ohashi H."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Ohashi H."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Kagami Y."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Kagami Y."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Mori K.J."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Mori K.J."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Murate T."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Murate T."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Tsushita K."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/author"Tsushita K."xsd:string
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8417953http://purl.uniprot.org/core/date"1993"xsd:gYear
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