| http://purl.uniprot.org/citations/8428657 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8428657 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8428657 | http://www.w3.org/2000/01/rdf-schema#comment | "A fragment of the cDNA encoding a rat valyl-tRNA synthetase (TrsVal)-like protein was cloned from a rat cDNA library in lambda gt11 using an oligodeoxyribonucleotide (oligo) probe. Three independent plaque clones containing the human TrsVal cDNA were then isolated from a lambda gt10 human erythroleukemia cDNA library using the rat cDNA fragment as the hybridization probe. Sequence analyses of the cDNA fragments provided a 3.2-kb sequence with an open reading frame that contained the 'HIGH' synthetase signature sequence and the tRNA 3'-end-binding motif, KMSKS, and putative Val-binding motif, EWCISRQ. The sequence was extended to the 3' end of the cDNA by the polymerase chain reaction using an internal primer and an oligo(dT) adapter. The deduced 1051-amino-acid sequence shares 65% identity with yeast TrsVal, and contains a highly basic N-terminal region, a newly evolved protease-sensitive region in sequence close to the C terminus, and several sites for protein kinase C phosphorylation. A 3-kb cDNA fragment was sub-cloned into plasmid pSVL and expressed in COS-7 cells; up to a sevenfold increase in TrsVal activity was obtained. These results confirm the cloning and sequencing of a human TrsVal-encoding cDNA."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(93)90122-j"xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(93)90122-j"xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Wood L."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Wood L."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Yang D.C.H."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Yang D.C.H."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Vogeli G."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Vogeli G."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Donovan D."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Donovan D."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Vilalta A."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/author | "Vilalta A."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/pages | "181-186"xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/pages | "181-186"xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/title | "Cloning, sequencing and expression of a cDNA encoding mammalian valyl-tRNA synthetase."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/title | "Cloning, sequencing and expression of a cDNA encoding mammalian valyl-tRNA synthetase."xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/volume | "123"xsd:string |
| http://purl.uniprot.org/citations/8428657 | http://purl.uniprot.org/core/volume | "123"xsd:string |