| http://purl.uniprot.org/citations/8446108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8446108 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8446108 | http://www.w3.org/2000/01/rdf-schema#comment | "An abundant 37-kDa protein, which comprises up to 30% of the soluble proteins of the ovary, has been found to have 20 alpha-hydroxysteroid dehydrogenase (20 alpha HSD) activity. The steroidogenic enzyme 20 alpha HSD regulates the conversion of progesterone to 20 alpha-hydroxyprogesterone in many mammalian species. Complimentary DNA clones encoding a unique and abundant 20 alpha HSD were isolated from a mature rabbit ovary library using guinea pig antisera generated to the purified 37-kDa protein and from a 5' EcoRI fragment from the initial positive clone. A full-length cDNA clone of 1217 basepairs encoding a 323-amino acid protein with an estimated mol wt of 37 kilodaltons was obtained. Amino acid sequence data indicate a similarity to human chlordecone reductase, bovine lung prostaglandin F synthase, human aldose reductase, human aldehyde reductase, and frog lens rho-crystallin, placing rabbit ovarian 20 alpha HSD in the aldo-keto reductase family of proteins. Northern blot analysis demonstrated a 1.2-kilobase mRNA in the interstitial tissue of mature rabbit ovaries and, to a lesser extent, in corpora luteal tissue. 20 alpha HSD was expressed in bacteria as a recombinant protein and was shown to possess enzymatic activity, preferring NADP as a cofactor. These studies demonstrate that an abundant ovarian protein belonging to the superfamily of NADP-dependent aldo-keto reductases has 20 alpha HSD activity. This is the first example of an abundant crystallin-related protein with known enzymatic activity in a tissue other than the lens."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.org/dc/terms/identifier | "doi:10.1210/mend.7.1.8446108"xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.org/dc/terms/identifier | "doi:10.1210/mend.7.1.8446108"xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Cook R.G."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Cook R.G."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Dunbar B.S."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Dunbar B.S."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Lacy W.R."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Lacy W.R."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Washenick K.J."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/author | "Washenick K.J."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/name | "Mol. Endocrinol."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/name | "Mol. Endocrinol."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/pages | "58-66"xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/pages | "58-66"xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/title | "Molecular cloning and expression of an abundant rabbit ovarian protein with 20 alpha-hydroxysteroid dehydrogenase activity."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/title | "Molecular cloning and expression of an abundant rabbit ovarian protein with 20 alpha-hydroxysteroid dehydrogenase activity."xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/8446108 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8446108 |
| http://purl.uniprot.org/citations/8446108 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8446108 |