| http://purl.uniprot.org/citations/8457543 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8457543 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8457543 | http://www.w3.org/2000/01/rdf-schema#comment | "The venom of the scorpion Androctonus mauretanicus mauretanicus contains a toxin, P05, which is structurally and functionally similar to scorpion leiurotoxin I (87% sequence identity), a blocker of the apamin-sensitive Ca(2+)-activated K+ channels. It is a 31-residue polypeptide cross-linked by three disulfide bridges. A C-terminal carboxyl-amidated analog of P05 (sP05-NH2) was chemically synthesized by the solid-phase technique and fully characterized. Toxicity assays in vivo established that sP05-NH2, like native P05, is a potent and lethal neurotoxic agent in mice (LD50 of 20 ng per mouse). Pharmacological assays in vitro however showed that, unlike P05 which has a binding affinity of 2 x 10(-11) M, sP05-NH2 apparently binds irreversibly to the apamin receptor. Iodination at the C-terminal His gave diiodo-sP05-NH2, which had a binding affinity similar to that of native P05. The disulfide bridge pairings were chemically determined for sP05-NH2 and thereby deduced for P05 and leiurotoxin I: linkages were between Cys3 and Cys21, Cys8 and Cys26, and Cys12 and Cys28. Molecular dynamics refinement of P05 also using data from leiurotoxin I suggests that P05 is mainly composed of a double-stranded, antiparallel beta-sheet (from Leu18 to Val29) linked to an alpha-helix (from Arg6 to Gly16) by two disulfides (Cys8-Cys26 and Cys12-Cys28) and to an extended fragment (from Thr1 to Leu5) by the third disulfide (Cys3-Cys21). In agreement with the model, circular dichroism analysis of sP05-NH2 showed that the toxin structure is highly rigid.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00062a005"xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00062a005"xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Rochat H."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Rochat H."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "van Rietschoten J."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "van Rietschoten J."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Darbon H."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Darbon H."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Benslimane A."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Benslimane A."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Mabrouk K."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Mabrouk K."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Sabatier J.-M."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Sabatier J.-M."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Martin-Eauclaire M.-F."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Martin-Eauclaire M.-F."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Zerrouk H."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/author | "Zerrouk H."xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8457543 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |