| http://purl.uniprot.org/citations/8477184 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8477184 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8477184 | http://www.w3.org/2000/01/rdf-schema#comment | "Sequence-specific 1H and 15N resonance assignments have been made for all 145 non-prolyl residues and for the flavin cofactor in oxidized Desulfovibrio vulgaris flavodoxin. Assignments were obtained by recording and analyzing 1H-15N heteronuclear three-dimensional NMR experiments on uniformly 15N-enriched protein, pH 6.5, at 300 K. Many of the side-chain resonances have also been assigned. Observed medium-and long-range NOEs, in combination with 3JNH alpha coupling constants and 1HN exchange data, indicate that the secondary structure consists of a five-stranded parallel beta-sheet and four alpha-helices, with a topology identical to that determined previously by X-ray crystallographic methods. One helix, which is distorted in the X-ray structure, is non-regular in solution as well. Several protein-flavin NOEs, which serve to dock the flavin ligand to its binding site, have also been identified. Based on fast-exchange into 2H2O, the 1HN3 proton of the isoalloxazine ring is solvent accessible and not strongly hydrogen-bonded in the flavin binding site, in contrast to what has been observed in several other flavodoxins. The resonance assignments presented here can form the basis for assigning single-site mutant flavodoxins and for correlating structural differences between wild-type and mutant flavodoxins with altered redox potentials."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00178258"xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00178258"xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Euvrard A."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Euvrard A."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Kloosterman D.A."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Kloosterman D.A."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Scahill T.A."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Scahill T.A."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Stockman B.J."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Stockman B.J."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Swenson R.P."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/author | "Swenson R.P."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/name | "J. Biomol. NMR"xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/name | "J. Biomol. NMR"xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/pages | "133-149"xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/pages | "133-149"xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/title | "1H and 15N resonance assignments and solution secondary structure of oxidized Desulfovibrio vulgaris flavodoxin determined by heteronuclear three-dimensional NMR spectroscopy."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/title | "1H and 15N resonance assignments and solution secondary structure of oxidized Desulfovibrio vulgaris flavodoxin determined by heteronuclear three-dimensional NMR spectroscopy."xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/8477184 | http://purl.uniprot.org/core/volume | "3"xsd:string |