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http://purl.uniprot.org/citations/8486659http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8486659http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8486659http://www.w3.org/2000/01/rdf-schema#comment"The cDNA for human beta-arrestin-1 was cloned by polymerase chain reaction (PCR) and identified based on its remarkably high amino acid identity (98.6%) with the bovine sequence. Two alternatively spliced isoforms of human beta-arrestin-1, differing only in the presence or absence of 24 base pairs/8 amino acids within the sequence, were identified and called beta-arrestin-1A and beta-arrestin-1B, respectively. Both isoforms were found in all tissues tested. Southern blot analysis revealed the existence of a single gene for beta-arrestin-1, suggesting that the two isoforms are generated by alternative mRNA splicing. The possible presence of similar isoforms was investigated for the other members of the arrestin/beta-arrestin gene family by PCR. Two isoforms of arrestin were revealed in bovine peripheral blood leukocytes. The expression of beta-arrestin-1 was studied in several human tissues and cell types. High levels of beta-arrestin-1 mRNA and immunoreactivity were found in peripheral blood leukocytes. The possible regulation of the expression of beta-arrestin-1 was also investigated. Our work documents for the first time that the expression of beta-arrestin-1 is modulated by intracellular cAMP. Using two cell types, human endothelial cells and smooth muscle cells, we found that 6-8-h treatments with the cAMP-inducing agents cholera toxin, forskolin, iloprost, and isoproterenol raised beta-arrestin-1 mRNA by 2-4-fold. Forskolin preferentially increased beta-arrestin-1A expression in smooth muscle cells, as assessed by PCR. beta-Arrestin-1 immunoreactivity was 2-3-fold higher in smooth muscle cells exposed to forskolin for 8 h, compared with untreated controls. We conclude that (i) the finding of alternatively spliced isoforms of beta-arrestin-1 and arrestin documents a novel mechanism to generate diversity within the arrestin/beta-arrestin gene family; (ii) the abundant expression of beta-arrestin-1 in peripheral blood leukocytes further supports our previous suggestion of a major role for the beta ARK/beta-arrestin system in regulating receptor-mediated immune functions; (iii) the increased expression of beta-arrestin-1 by cAMP suggests a new mechanism for the regulation of receptor-mediated responses."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)98412-7"xsd:string
http://purl.uniprot.org/citations/8486659http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(18)98412-7"xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Rotilio D."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Rotilio D."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Sallese M."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Sallese M."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Ambrosini G."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Ambrosini G."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Parruti G."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Parruti G."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"de Blasi A."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"de Blasi A."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Masini M."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Masini M."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Peracchia F."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/author"Peracchia F."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/date"1993"xsd:gYear
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/pages"9753-9761"xsd:string
http://purl.uniprot.org/citations/8486659http://purl.uniprot.org/core/pages"9753-9761"xsd:string
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