| http://purl.uniprot.org/citations/8506296 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8506296 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8506296 | http://www.w3.org/2000/01/rdf-schema#comment | "Human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA clones were isolated from an HL-60 cell cDNA library by using a reverse transcription/polymerase chain reaction-generated human cDNA probe. The 24-hydroxylase cDNA consists of a 1539-bp open reading frame encoding a 513-amino acid polypeptide. Protein sequence analysis shows that the human 24-hydroxylase is 90% homologous (82% identical) to that of the rat, with 100% homology in the 21-amino acid heme-binding region. Northern blot analysis showed that the 24-hydroxylase cDNA probe hybridized to a 3.4-kb mRNA species. Treatment of HL-60 cells with 0.1 microM 1,25-dihydroxyvitamin D3 for 24 hr produced a 30-fold increase in the 24-hydroxylase mRNA level. This result is consistent with previous studies in the same cell line, in which 24-hydroxylase activity was elevated to a maximum in 24 hr by a similar treatment with 1,25-dihydroxyvitamin D3. To verify the identity of these isolated cDNA clones, two polymerase chain reaction-amplified human 24-hydroxylase cDNA fragments containing the entire coding region were used to produce 24-hydroxylase enzyme activity in two genetic expression systems. Transient levels of 24-hydroxylase activity were measured in transfected mammalian COS-1 cells and in recombinant baculovirus-infected Spodoptera frugiperda (Sf21) insect cells."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.90.10.4543"xsd:string |
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| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/author | "Deluca H.F."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/author | "Deluca H.F."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/author | "Chen K.-S."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/author | "Chen K.-S."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/author | "Prahl J.M."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/author | "Prahl J.M."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/pages | "4543-4547"xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/pages | "4543-4547"xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/title | "Isolation and expression of human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/title | "Isolation and expression of human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA."xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/volume | "90"xsd:string |
| http://purl.uniprot.org/citations/8506296 | http://purl.uniprot.org/core/volume | "90"xsd:string |
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| http://purl.uniprot.org/citations/8506296 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8506296 |
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| http://purl.uniprot.org/citations/8506296 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8506296 |