| http://purl.uniprot.org/citations/8527927 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8527927 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8527927 | http://www.w3.org/2000/01/rdf-schema#comment | "2'-Deoxyuridylate hydroxymethylase (dUMP-hmase) from phage SPO1 has been cloned and expressed in Escherichia coli. In crude extracts, the enzyme represents about 25% of the soluble protein and has a higher specific activity than the most purified preparation yet reported. The enzyme was purified to homogeneity by ion-exchange and hydrophobic chromatography. The subunits of dUMP-hmase are 45 kDa by SDS-PAGE and form dimers with a molecular mass of 89.2 kDa by analytical centrifugation. In addition to the normal reaction, dUMP-hmase catalyzes the 5,10-methylene-5,6,7,8-tetrahydrofolate (CH2H4folate)-independent tritium exchange of [5-3H]dUMP for protons of water and dehalogenation of 5-bromo-2'-deoxy-uridine-5'-monophosphate; the enzyme also forms a covalent binary adduct with pyridoxal 5'-monophosphate and a covalent ternary complex with 5-fluoro-2'-deoxyuridine-5'-monophosphate and CH2H4folate. Folic acid inhibits the tritium release catalyzed by dUMP-hmase in the presence of cofactor but has no effect on the catalysis of cofactor-independent tritium exchange."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.org/dc/terms/identifier | "doi:10.1006/prep.1995.1057"xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.org/dc/terms/identifier | "doi:10.1006/prep.1995.1057"xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/author | "Santi D.V."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/author | "Santi D.V."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/author | "Livi L.L."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/author | "Livi L.L."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/author | "Schellenberger U."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/author | "Schellenberger U."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/pages | "423-430"xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/pages | "423-430"xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/title | "Cloning, expression, purification, and characterization of 2'-deoxyuridylate hydroxymethylase from phage SPO1."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/title | "Cloning, expression, purification, and characterization of 2'-deoxyuridylate hydroxymethylase from phage SPO1."xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/8527927 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8527927 |
| http://purl.uniprot.org/citations/8527927 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8527927 |
| http://purl.uniprot.org/citations/8527927 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8527927 |
| http://purl.uniprot.org/citations/8527927 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8527927 |