| http://purl.uniprot.org/citations/8602245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8602245 | http://www.w3.org/2000/01/rdf-schema#comment | "Kinesin is a two-headed motor protein that powers organelle transport along microtubules. Many ATP molecules are hydrolysed by kinesin for each diffusional encounter with the microtubule. Here we report the development of a new assay in which the processive movement of individual fluorescently labelled kinesin molecules along a microtubule can be visualized directly; this observation is achieved by low-background total internal reflection fluorescence microscopy in the absence of attachment of the motor to a cargo (for example, an organelle or bead). The average distance travelled after a binding encounter with a microtubule is 600 nm, which reflects a approximately 1% probability of detachment per mechanical cycle. Surprisingly, processive movement could still be observed at salt concentrations as high as 0.3 M NaCl. Truncated kinesin molecules having only a single motor domain do not show detectable processive movement, which is consistent with a model in which kinesin's two force-generating heads operate by a hand-over-hand mechanism."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.org/dc/terms/identifier | "doi:10.1038/380451a0"xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/author | "Pierce D.W."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/author | "Vale R.D."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/author | "Harada Y."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/author | "Yanagida T."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/author | "Romberg L."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/author | "Funatsu T."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/pages | "451-453"xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/title | "Direct observation of single kinesin molecules moving along microtubules."xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://purl.uniprot.org/core/volume | "380"xsd:string |
| http://purl.uniprot.org/citations/8602245 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8602245 |
| http://purl.uniprot.org/citations/8602245 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8602245 |
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| http://purl.uniprot.org/uniprot/#_A6NNZ2-mappedCitation-8602245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8602245 |
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| http://purl.uniprot.org/uniprot/#_O14782-mappedCitation-8602245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8602245 |
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| http://purl.uniprot.org/uniprot/#_Q13509-mappedCitation-8602245 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/8602245 |
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